ASSESSMENT OF CONTROVERSIAL RISK FACTORS IN DEVELOPMENT OF BREAST CANCER: A STUDY FROM LOCAL POPULATION

A Malik; K Hafeez; W Nazar; M Naeem; I Ali; Q Ali; Ahmed; Z Mujtaba; MA Rana; MM Hafeez

doi:10.54112/bcsrj.v2021i1.49

Authors

A Malik Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
K Hafeez Nawaz Sharif Medical College, Gujrat-Pakistan
W Nazar Respiratory Reg., Cavan General Hospital, Ireland
M Naeem Peadiatric Medicine, Al Aleem Medical college, Gulab devi hospital, Lahore
I Ali Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
Q Ali Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
Ahmed Institute of Agricultural Sciences, University of the Punjab Lahore, Lahore, Pakistan
Z Mujtaba Institute of Agricultural Extension, Education and Rural Development, University of Agriculture, Faisalabad, Punjab, Pakistan
MA Rana Intensive Care Unit, Bahria International Hospital Lahore-Pakistan
MM Hafeez Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2021i1.49

Keywords:

Breast Cancer, Vitamin D deficiency, Reactive Oxygen Species, 8-hydroxydeoxyguanosine, thyroid, hormone

Abstract

Breast cancer is the most common cause of death worldwide in women. Several predisposing risk factors have been identified making its incidence constantly rising. The aim of the current study was to analyze thyroid hormone, vitamin D, and 8-hydroxydeoxyguanosine (8-OHdG) as risk factors in the onset of breast cancer. In the present case and control study, a total of two hundred and seventy-four (n=274) participants were included after taken the informed consent individually. The participants were further stratified into two groups. Group A consisted of one hundred and thirty-seven age and sex-matched healthy individuals presented at OPD whereas group B had one hundred and thirty-seven diagnosed cases of breast cancer. Serum vitamin D, thyroid hormone, and 8-OHdG were measured by commercially available ELISA kits by using the blood samples drawn from the study population. Data were analyzed with SPSS V21.0 and a p-value less than 0.05 considered statistically significant. Cases and control were comparable in terms of age, BMI, and gender distribution. Vitamin D deficiency was present in 78.4% of cases and 63.5% of control. (OR 3.83 95% CI 1.38 -10.5) 8-OHdG was present in 56.8% and 11.1% in cases and control respectively (P 0.001). Similarly, MDA was found positive in 67.6% of cases and 21.6% of control (P 0.001). Vitamin D and thyroid hormone deficiency and elevated levels of reactive oxygen species (ROS) are supposed to associate with increased risk in the development of breast cancer.

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References

Begum, N. (2018). Breast cancer in Pakistan: a looming epidemic. J Coll Physicians Surg Pak 28, 87-8. DOI: https://doi.org/10.29271/jcpsp.2018.02.87

Chen, C.-Y., Tsai, M.-M., Chi, H.-C., and Lin, K.-H. (2013). Biological significance of a thyroid hormone-regulated secretome. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1834, 2271-2284. DOI: https://doi.org/10.1016/j.bbapap.2013.02.016

Chi, H.-C., Chen, S.-L., Cheng, Y.-H., Lin, T.-K., Tsai, C.-Y., Tsai, M.-M., Lin, Y.-H., Huang, Y.-H., and Lin, K.-H. (2016). Chemotherapy resistance and metastasis-promoting effects of thyroid hormone in hepatocarcinoma cells are mediated by suppression of FoxO1 and Bim pathway. Cell death & disease 7, e2324-e2324. DOI: https://doi.org/10.1038/cddis.2016.227

Chi, H., Chen, S., Liao, C., Liao, C., Tsai, M., Lin, Y., Huang, Y., Yeh, C., Wu, S., and Tseng, Y. (2012). Thyroid hormone receptors promote metastasis of human hepatoma cells via regulation of TRAIL. Cell Death & Differentiation 19, 1802-1814. DOI: https://doi.org/10.1038/cdd.2012.58

Davis, P. J., Davis, F. B., Lin, H.-Y., Mousa, S. A., Zhou, M., and Luidens, M. K. (2009). Translational implications of nongenomic actions of thyroid hormone initiated at its integrin receptor. American Journal of Physiology-Endocrinology and Metabolism 297, E1238-E1246. DOI: https://doi.org/10.1152/ajpendo.00480.2009

DeSantis, C. E., Ma, J., Goding Sauer, A., Newman, L. A., and Jemal, A. (2017). Breast cancer statistics, 2017, racial disparity in mortality by state. CA: a cancer journal for clinicians 67, 439-448. DOI: https://doi.org/10.3322/caac.21412

Dong, L., Lu, J., Zhao, B., Wang, W., and Zhao, Y. (2018). Review of the possible association between thyroid and breast carcinoma. World journal of surgical oncology 16, 130. DOI: https://doi.org/10.1186/s12957-018-1436-0

Feng, Y., Spezia, M., Huang, S., Yuan, C., Zeng, Z., Zhang, L., Ji, X., Liu, W., Huang, B., and Luo, W. (2018). Breast cancer development and progression: Risk factors, cancer stem cells, signaling pathways, genomics, and molecular pathogenesis. Genes & diseases 5, 77-106. DOI: https://doi.org/10.1016/j.gendis.2018.05.001

Glover, A., Zhao, J., Ip, J., Lee, J., Robinson, B., Gill, A., Soon, P., and Sidhu, S. (2015). Long noncoding RNA profiles of adrenocortical cancer can be used to predict recurrence. Endocrine-Related Cancer 22, 99-109. DOI: https://doi.org/10.1530/ERC-14-0457

Hall, L. C., Salazar, E. P., Kane, S. R., and Liu, N. (2008). Effects of thyroid hormones on human breast cancer cell proliferation. The Journal of steroid biochemistry and molecular biology 109, 57-66. DOI: https://doi.org/10.1016/j.jsbmb.2007.12.008

Hernando, V.-U., Andry, M.-M., Virginia, P.-F. M., and Valentina, A. (2020). Vitamin D nutritional status in the adult population in Colombia–An analytical cross-sectional study. Heliyon 6, e03479. DOI: https://doi.org/10.1016/j.heliyon.2020.e03479

Joo, J. H., and Jetten, A. M. (2010). Molecular mechanisms involved in farnesol-induced apoptosis. Cancer letters 287, 123-135. DOI: https://doi.org/10.1016/j.canlet.2009.05.015

Journy, N. M., Bernier, M.-O., Doody, M. M., Alexander, B. H., Linet, M. S., and Kitahara, C. M. (2017). Hyperthyroidism, hypothyroidism, and cause-specific mortality in a large cohort of women. Thyroid 27, 1001-1010. DOI: https://doi.org/10.1089/thy.2017.0063

Kaminskyy, V., Surova, O., Piskunova, T., Zborovskaya, I., Tchevkina, E., Andera, L., and Zhivotovsky, B. (2013). Upregulation of c-FLIP-short in response to TRAIL promotes survival of NSCLC cells, which could be suppressed by inhibition of Ca 2+/calmodulin signaling. Cell death & disease 4, e522-e522. DOI: https://doi.org/10.1038/cddis.2013.51

Khan, S. R., Chaker, L., Ruiter, R., Aerts, J. G., Hofman, A., Dehghan, A., Franco, O. H., Stricker, B. H., and Peeters, R. P. (2016). Thyroid function and cancer risk: the Rotterdam study. The Journal of Clinical Endocrinology & Metabolism 101, 5030-5036. DOI: https://doi.org/10.1210/jc.2016-2104

Kitahara, C. M., De Gonzalez, A. B., Bouville, A., Brill, A. B., Doody, M. M., Melo, D. R., Simon, S. L., Sosa, J. A., Tulchinsky, M., and Villoing, D. (2019). Association of radioactive iodine treatment with cancer mortality in patients with hyperthyroidism. JAMA internal medicine 179, 1034-1042. DOI: https://doi.org/10.1001/jamainternmed.2019.0981

Kleine, C.-E., Obi, Y., Streja, E., Hsiung, J.-T., Park, C., Holick, M. F., and Kalantar-Zadeh, K. (2019). Seasonal variation of serum 25-hydroxyvitamin D and parameters of bone and mineral disorder in dialysis patients. Bone 124, 158-165. DOI: https://doi.org/10.1016/j.bone.2019.03.003

Krashin, E., Piekiełko-Witkowska, A., Ellis, M., and Ashur-Fabian, O. (2019). Thyroid hormones and cancer: A comprehensive review of preclinical and clinical studies. Frontiers in endocrinology 10, 59. DOI: https://doi.org/10.3389/fendo.2019.00059

Kuijpens, J. L., Nyklíčtek, I., Louwman, M. W., Weetman, T. A., Pop, V. J., and Coebergh, J.-W. W. (2005). Hypothyroidism might be related to breast cancer in post-menopausal women. Thyroid 15, 1253-1259. DOI: https://doi.org/10.1089/thy.2005.15.1253

Lee, J. D., Cai, Q., Shu, X. O., and Nechuta, S. J. (2017). The role of biomarkers of oxidative stress in breast cancer risk and prognosis: A systematic review of the epidemiologic literature. Journal of Women's Health 26, 467-482. DOI: https://doi.org/10.1089/jwh.2016.5973

Lin, Y.-H., Wu, M.-H., Liao, C.-J., Huang, Y.-H., Chi, H.-C., Wu, S.-M., Chen, C.-Y., Tseng, Y.-H., Tsai, C.-Y., and Chung, I.-H. (2015). Repression of microRNA-130b by thyroid hormone enhances cell motility. Journal of Hepatology 62, 1328-1340. DOI: https://doi.org/10.1016/j.jhep.2014.12.035

Lin, Y., Liao, C., Huang, Y., Wu, M., Chi, H., Wu, S., Chen, C., Tseng, Y., Tsai, C., and Chung, I. (2013). Thyroid hormone receptor represses miR-17 expression to enhance tumor metastasis in human hepatoma cells. Oncogene 32, 4509-4518. DOI: https://doi.org/10.1038/onc.2013.309

Majeed, W., Aslam, B., Javed, I., Khaliq, T., Muhammad, F., Ali, A., and Raza, A. (2014). Breast cancer: major risk factors and recent developments in treatment. Asian Pac J Cancer Prev 15, 3353-3358. DOI: https://doi.org/10.7314/APJCP.2014.15.8.3353

Parva, N. R., Tadepalli, S., Singh, P., Qian, A., Joshi, R., Kandala, H., Nookala, V. K., and Cheriyath, P. (2018). Prevalence of vitamin D deficiency and associated risk factors in the US population (2011-2012). Cureus 10. DOI: https://doi.org/10.7759/cureus.2741

Pellegriti, G., Frasca, F., Regalbuto, C., Squatrito, S., and Vigneri, R. (2013). Worldwide increasing incidence of thyroid cancer: update on epidemiology and risk factors. Journal of cancer epidemiology 2013. DOI: https://doi.org/10.1155/2013/965212

Qi, P., Wang, L., Zhou, B., Yao, W., Xu, S., Zhou, Y., and Xie, Z. (2015). Associations of miRNA polymorphisms and expression levels with breast cancer risk in the Chinese population. Genet Mol Res 14, 6289-6296. DOI: https://doi.org/10.4238/2015.June.11.2

Sarwar, M. R., and Saqib, A. (2017). Cancer prevalence, incidence and mortality rates in Pakistan in 2012. Cogent Medicine 4, 1288773. DOI: https://doi.org/10.1080/2331205X.2017.1288773

Silva, C. O., Pinho, J. O., Lopes, J. M., Almeida, A. J., Gaspar, M. M., and Reis, C. (2019). Current trends in cancer nanotheranostics: metallic, polymeric, and lipid-based systems. Pharmaceutics 11, 22. DOI: https://doi.org/10.3390/pharmaceutics11010022

Søgaard, M., Farkas, D. K., Ehrenstein, V., Jørgensen, J., Dekkers, O. M., and Sørensen, H. T. (2016). Hypothyroidism and hyperthyroidism and breast cancer risk: a nationwide cohort study. European journal of endocrinology 174, 409. DOI: https://doi.org/10.1530/EJE-15-0989

Sun, Y.-S., Zhao, Z., Yang, Z.-N., Xu, F., Lu, H.-J., Zhu, Z.-Y., Shi, W., Jiang, J., Yao, P.-P., and Zhu, H.-P. (2017). Risk factors and preventions of breast cancer. International journal of biological sciences 13, 1387. DOI: https://doi.org/10.7150/ijbs.21635

Tosovic, A., Becker, C., Bondeson, A. G., Bondeson, L., Ericsson, U. B., Malm, J., and Manjer, J. (2012). Prospectively measured thyroid hormones and thyroid peroxidase antibodies in relation to breast cancer risk. International journal of cancer 131, 2126-2133. DOI: https://doi.org/10.1002/ijc.27470

Tseng, F.-Y., Lin, W.-Y., Li, C.-I., Li, T.-C., Lin, C.-C., and Huang, K.-C. (2015). Subclinical hypothyroidism is associated with increased risk for cancer mortality in adult Taiwanese—a 10 years population-based cohort. PloS one 10, e0122955. DOI: https://doi.org/10.1371/journal.pone.0122955

Wang, B., Song, R., He, W., Yao, Q., Li, Q., Jia, X., and Zhang, J.-a. (2018). Sex differences in the associations of obesity with hypothyroidism and thyroid autoimmunity among Chinese adults. Frontiers in physiology 9, 1397. DOI: https://doi.org/10.3389/fphys.2018.01397

Wang, H., and Chen, L. (2013). Tumor microenviroment and hepatocellular carcinoma metastasis. Journal of gastroenterology and hepatology 28, 43-48. DOI: https://doi.org/10.1111/jgh.12091

Weng, C.-H., Chen, Y.-H., Lin, C.-H., Luo, X., and Lin, T.-H. (2018). Thyroid disorders and breast cancer risk in Asian population: a nationwide population-based case–control study in Taiwan. BMJ open 8. DOI: https://doi.org/10.1136/bmjopen-2017-020194

Wolf, S. T., Jablonski, N. G., Ferguson, S. B., Alexander, L. M., and Kenney, W. L. (2020). Four weeks of vitamin D supplementation improves nitric oxide-mediated microvascular function in college-aged African Americans. American Journal of Physiology-Heart and Circulatory Physiology 319, H906-H914. DOI: https://doi.org/10.1152/ajpheart.00631.2020

Wu, J., Yang, C., Guo, C., Li, X., Yang, N., Zhao, L., Hang, H., Liu, S., Chu, P., and Sun, Z. (2016). SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Chemico-biological interactions 244, 94-104. DOI: https://doi.org/10.1016/j.cbi.2015.11.013

Wu, S.-M., Cheng, W.-L., Lin, C. D., and Lin, K.-H. (2013). Thyroid hormone actions in liver cancer. Cellular and Molecular Life Sciences 70, 1915-1936. DOI: https://doi.org/10.1007/s00018-012-1146-7

Zülch, K. J. (2013). "Brain tumors: their biology and pathology," Springer-Verlag.