• B Hameed Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
  • Q Ali Institute of Biotechnology and Molecular Biology, The University of Lahore, Lahore
  • MM Hafeez Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
  • A Malik Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan



Citrullus colocynthis, antifungal, antibacterial, fruit pulp, seed, root, zone of inhibition


Gastrointestinal, skin, pulmonary and cardiovascular problems have been reported all over the world on massive scale. The treatment of these problems has become tough due to genetically modified bacterial strains and fungal infections. The present studied was conducted to evaluate the antimicrobial activities of Citrullus colocynthis plant extract. The ethanolic extract of dried fruit pulp, seed, and root was evaluated with respect to anti-bacterial and anti-fungal properties. The anti-microbial profile studied against four bacterial strains (2 Gram negative and 2 Gram positive) while anti-fungal profile studied against four fungal species. All the bacterial and fungal strains used in the study showed sensitivities against the respective extracts. The zones of inhibition ranged between 7 mm to 23 mm, and 6 mm to 23 mm in against bacterial and fungal strains, respectively. The extract of seed found to be less effective against both the organisms. It was suggested from our study that the extract of Citrullus colocynthis may be used in medicines to cure bacterial diseases.


Download data is not yet available.


Bernard, S. A., and Olayinka, O. A. (2010). Search for a novel antioxidant, anti-inflammatory/analgesic or anti-proliferative drug: Cucurbitacins hold the ace. Journal of Medicinal Plants Research 4, 2821-2826.

Bhasin, A., Singh, S., and Garg, R. (2020). Nutritional and medical importance of Citrullus colocynthis-A review. Plant Archives 20, 3400-3406.

Dastmalchi, K., Dorman, H. D., Koşar, M., and Hiltunen, R. (2007). Chemical composition and in vitro antioxidant evaluation of a water-soluble Moldavian balm (Dracocephalum moldavica L.) extract. LWT-Food Science and Technology 40, 239-248.

Davidovich‐Rikanati, R., Shalev, L., Baranes, N., Meir, A., Itkin, M., Cohen, S., Zimbler, K., Portnoy, V., Ebizuka, Y., and Shibuya, M. (2015). Recombinant yeast as a functional tool for understanding bitterness and cucurbitacin biosynthesis in watermelon (Citrullus spp.). Yeast 32, 103-114.

Dhakad, P. K., Sharma, P. K., and Kumar, S. (2017). A review on phytochemical studies and biological potential of Citrullus colocynthis (L.) Schrad (Cucurbitaceae). J Bioeng Biosci 5, 55-64.

Dhanotia, R., Chauhan, N. S., Saraf, D. K., and Dixit, V. K. (2011). Effect of Citrullus colocynthis Schrad fruits on testosterone-induced alopecia. Natural product research 25, 1432-1443.

Gurudeeban, S., Satyavani, K., and Ramanathan, T. (2010). Bitter apple (Citrullus colocynthis): An overview of chemical composition and biomedical potentials. Asian Journal of Plant Sciences 9, 394.

Hussain, A. I., Rathore, H. A., Sattar, M. Z., Chatha, S. A., Sarker, S. D., and Gilani, A. H. (2014). Citrullus colocynthis (L.) Schrad (bitter apple fruit): A review of its phytochemistry, pharmacology, traditional uses and nutritional potential. Journal of ethnopharmacology 155, 54-66.

Jayaraman, R., Shivakumar, A., Anitha, T., Joshi, V., and Palei, N. (2009). Antidiabetic effect of petroleum ether extract of Citrullus colocynthis fruits against streptozotocin-induced hyperglycemic rats. Rom J Biol Plant Biol 4, 127-34.

Kapoor, M., Kaur, N., Sharma, C., Kaur, G., Kaur, R., Batra, K., and Rani, J. (2020). Citrullus colocynthis an Important Plant in Indian Traditional System of Medicine. Pharmacognosy Reviews 14, 23.

Kaushik, U., Aeri, V., and Mir, S. R. (2015). Cucurbitacins–an insight into medicinal leads from nature. Pharmacognosy reviews 9, 12.

Kouadri, I., and Satha, H. (2018). Extraction and characterization of cellulose and cellulose nanofibers from Citrullus colocynthis seeds. Industrial Crops and Products 124, 787-796.

Mehrzadi, S., Shojaii, A., Pur, S. A., and Motevalian, M. (2016). Anticonvulsant activity of hydroalcoholic extract of Citrullus colocynthis fruit: involvement of benzodiazepine and opioid receptors. Journal of evidence-based complementary & alternative medicine 21, NP31-NP35.

Onyeji, C. O., Igbinoba, S. I., and Olayiwola, G. (2017). Therapeutic potentials and cytochrome P450-mediated interactions involving herbal products indicated for diabetes mellitus. Drug metabolism letters 11, 74-85.

Pravin, B., Tushar, D., Vijay, P., and Kishanchnad, K. (2013). Review on Citrullus colocynthis. Int. J. Res. Pharm. Chem 3, 46-53.

Rezai, M., Davoodi, A., Asori, M., and Azadbakht, M. (2017). Cytotoxic activity of citrullus colocynthis (L.) schrad fruit extract on gastric adenocarcinoma and breast cancer cell lines. Int J PhaInt J Pharm Sci Rev Res 45, 175-178.

Riaz, H., Chatha, S. A. S., Hussain, A. I., Bukhari, S. A., Hussain, S. M., and Zafar, K. (2015). Physico-chemical characterization of bitter apple (Citrullus colosynthis) seed oil and seed residue. Int. J. Biosci 6, 283-292.

Salehi, B., Capanoglu, E., Adrar, N., Catalkaya, G., Shaheen, S., Jaffer, M., Giri, L., Suyal, R., Jugran, A. K., and Calina, D. (2019). Cucurbits plants: A key emphasis to its pharmacological potential. Molecules 24, 1854.

Sharififar, F., Moshafi, M., Mansouri, S., Khodashenas, M., and Khoshnoodi, M. (2007). In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food control 18, 800-805.

Song, F., Dai, B., Zhang, H.-Y., Xie, J.-W., Gu, C.-Z., and Zhang, J. (2015). Two new cucurbitane-type triterpenoid saponins isolated from ethyl acetate extract of Citrullus colocynthis fruit. Journal of Asian natural products research 17, 813-818.

Yazit, S., Nemmiche, S., Amamou, F., Meziane, R., and Chabane-Sari, D. (2019). Anti-hyperlipidemic Effect of Fatty Acids Methyl Esters (FAMEs) of Citrullus colocynthis in High-Fat Diet Induced Obesity in Rats. Phytothérapie.




How to Cite

Hameed, B., Ali, Q., Hafeez, M., & Malik, A. (2020). ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF FRUIT, SEED AND ROOT EXTRACTS OF CITRULLUS COLOCYNTHIS PLANT. Biological and Clinical Sciences Research Journal, 2020(1).



Original Research Articles

Most read articles by the same author(s)

1 2 3 4 5 6 7 > >>