• Kamran Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
  • Q Ali Institute of Biotechnology and Molecular Biology, The University of Lahore, Lahore
  • A Malik Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan



chickpea, pea, green beans, mung bean, stress, biogas wastewater, zinc sulfate


The legumes are very important food crops, called pulses grown throughout the world for their grain which contains essential vitamins, carbohydrates, protein, fat, minerals, and dietary fiber. Chickpea (Cicer arietinum L.), pea (Pisum sativum L.), green mung bean (Vigna radiate L.), and black mung bean (Vigna mungo L.) is important pulse crops that belong to the family Fabaceae or Leguminosae. The present experiment was conducted at the greenhouse of Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore during the season of July to August of 2020 to study the effects of different treatments of ZnSO4 and biogas wastewater on the growth of chickpea, pea, green bean, and black bean. The experiment consisted of two treatment combinations comprising of two levels of ZnSO4 at 0.5M and 0.25M along with the two levels of biogas wastewater at 500ml and 250ml along with including the control group. It was observed from results that the performance of Chickpea, pea, green mung bean, and black mung bean genotypes were variable under heavy metal zinc sulfate treatment. The results suggested that the treatment of a higher concentration of 0.5M ZnSO4 was toxic as compared with 0.25M ZnSO4. The application of biogas wastewater was found relatively fit for the seedling growth of all of four pulse crop species. The results showed that there was a significant correlation among root length, shoot length, and leaf length under the application of different treatments. A higher genetic advance was reported for shoot length and root length which revealed that the selection of legumes may be fruitful to improve yield under stress conditions.


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How to Cite

Kamran, Ali, Q., & Malik, A. (2021). GENETIC EVALUATION OF LEGUME SPECIES UNDER HEAVY METAL AND BIOGAS WASTEWATER TREATMENTS. Biological and Clinical Sciences Research Journal, 2021(1).



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