• I ASHRAF Agriculture Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
  • M ALI Sustainable Development Study Centre, Government College University, Lahore, Pakistan
  • M ASIF Faculty of Agricultural Sciences, Department of Plant Pathology, University of the Punjab, Lahore, Pakistan
  • W ANWAR Faculty of Agricultural Sciences, Department of Plant Pathology, University of the Punjab, Lahore, Pakistan
  • A AKHTAR Faculty of Agricultural Sciences, Department of Plant Pathology, University of the Punjab, Lahore, Pakistan
  • HA ALI Faculty of Agricultural Sciences, Department of Entomology, University of the Punjab, Lahore. Pakistan
  • NT BUKHARI Department of Microbiology and Clinical Laboratory Sciences, Women University Swabi, Swabi. Pakistan
  • Q ALI Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, University of the Punjab, Lahore, Pakistan
  • M SHAFIQ Rawalpinidi Medical University, Tipu Road, Chamanzar Colony, Rawalpindi, Pakistan




Antifungal proteins, Bio-pesticides, Crude proteins, Thermal stability, biological formulations


Alternaria solani was isolated from infected tomato leaves, and Trametes versicolor from northern areas of Pakistan established mycelial culture on PDA. Crude Protein extract was prepared in 50mM Sodium phosphate buffer by macerating C. versicolor (T/C) mycelium. Extracted protein solution (Crude extract) was filtered and tested for antifungal activity by using different concentrations (0.5mg/ml, 1mg/ml, 1.5mg/ml, and 2mg/ml) against A. solani. A protein with antifungal potential was purified using the DEAE- Cellulose column followed by the gel filtration column of Superdex75. MIC was also observed for purified antifungal protein by using micro spectrophotometry. Results showed that T. versicolor has the potential to inhibit the fungal growth of A. solani. Crude extract with a concentration of 2mg/mL inhibited 83% of the growth of A. solani. However, only 47% of inhibition was observed in the case of 0.5mg/ml of protein extract. The protein having antifungal potential purified by anion exchange chromatography has an approximate size of 15 kDa. MIC for the purified protein is 200µg, which inhibited the 100% growth of A. solani.


Download data is not yet available.


Parroni, A., Bellabarba, A., Beccaccioli, M., Scarpari, M., Reverberi, M., & Infantino, A. (2019). Use of the secreted proteome of Trametes versicolor for controlling the cereal pathogen Fusarium langsethiae. International journal of molecular sciences, 20(17), 4167.

Alves, M. J., Ferreira, I. C., Froufe, H. J., Abreu, R. M. V., Martins, A., & Pintado, M. (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of applied microbiology, 115(2), 346-357.

Anupama, S., Manali, M., & Sonali, R. (2015). Antifungal activity of a fungal isolates against Pomegranate wilt pathogen Fusarium. International Journal of Current Microbiology and Applied Sciences, 4(Special Issue 2), 48-57.

Baig, M. N., Shahid, A. A., & Ali, M. (2015). In vitro assessment of extracts of the lingzhi or reishi medicinal mushroom, Ganoderma lucidum (higher basidiomycetes) against different plant pathogenic fungi. International journal of medicinal mushrooms, 17(4).

Broekaert, W. F., Terras, F. R., Cammue, B. P., & Vanderleyden, J. (1990). An automated quantitative assay for fungal growth inhibition. FEMS Microbiology Letters, 69(1-2), 55-59.

Cheng, K. F., & Leung, P. C. (2008). General review of polysaccharopeptides (PSP) from C. versicolor: Pharmacological and clinical studies. Cancer Therapy, 6, 117-130.

Chu, K. K., Ho, S. S., & Chow, A. H. (2002). Coriolus versicolor: a medicinal mushroom with promising immunotherapeutic values. The Journal of Clinical Pharmacology, 42(9), 976-984.

Engindeniz, S., & Cosar, G. O. (2013). An economic comparison of pesticide applications for processing and table tomatoes: A case study for Turkey. Journal of Plant Protection Research. 53(3):230–237. DOI: https://doi.org/10.2478/jppr-2013-0035

Fagade, O. E., & Oyelade, A. A. (2009). A comparative study of the antibacterial activities of some wood-decay fungi to synthetic antibiotic discs. Electronic Journal of Environmental, Agricultural and Food Chemistry, 8(3), 184-188.

Foolad, M. R., Merk, H. L., & Ashrafi, H. (2008). Genetics, genomics and breeding of late blight and early blight resistance in tomato. Critical Reviews in Plant Sciences, 27(2), 75-107.

Government of Pakistan. 2017. Agricultural Statistics of Pakistan 2016-2017. Islamabad, Ministry of Food, Agriculture and Co- Operatives.

Yan, J., Yuan, S. S., Jiang, L. L., Ye, X. J., Ng, T. B., & Wu, Z. J. (2015). Plant antifungal proteins and their applications in agriculture. Applied microbiology and biotechnology, 99(12), 4961-4981.

Li, F., Wen, H., Zhang, Y., Aa, M., & Liu, X. (2011). Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor. Science China Life Sciences, 54(4), 379-385.

Iqbal, J., & Nafisa, A. S. (2018). Cultural, morphological, molecular comparison and pathogenicity of Alternaria solani causing early blight disease in tomato. Mycopath, 15(1).

Ng, T. B. (1998). A review of research on the protein-bound polysaccharide (polysaccharopeptide, PSP) from the mushroom Coriolus versicolor (Basidiomycetes: Polyporaceae). General Pharmacology: The Vascular System, 30(1), 1-4.

Ofodile, L. N., Uma, N. U., Kokubun, T., Grayer, R. J., Ogundipe, O. T., & Simmonds, M. S. J. (2005). Antimicrobial activity of some Ganoderma species from Nigeria. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 19(4), 310-313.

Parthasarathy, S., Azizi, J. B., Ramanathan, S., Ismail, S., Sasidharan, S., Said, M. I. M., & Mansor, S. M. (2009). Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (Rubiaceae family) leaves. Molecules, 14(10), 3964-3974.

Pushpa, H., & Purushothama, K. B. (2010). Antimicrobial activity of Lyophyllum decastes an edible wild mushroom. World Journal of Agricultural Sciences, 6(5), 506-509.

Vidhyasekaran, L. (1983). Efficacy of fungicides on the control of Alternaria leaf spot of tomato. Madras Agriculture, 85, 122-123.

Saleem, M. Y., Akhtar, K. P., Iqbal, Q., Asghar, M., Hameed, A., & Shoaib, M. (2016). Development of tomato hybrids with multiple disease tolerance. Pak. J. Bot, 48(2), 771-778.

Singh, S. B., & Kuwar, S. (2005). Evaluation of native bio-agents against Alternaria brassicae causing Alternaria blight of mustard. Farm Science Journal, 14(2).:64.

Sivaprakasam, E., Kavitha, D., Balakumar, R., Sridhar, S., & Kumar, J. S. (2011). Antimicrobial activity of whole fruiting bodies of Trametes hirsuta (Wulf. Fr.) Pil. against some common pathogenic bacteria and fungus. International Journal of Pharmaceutical Sciences and Drug Research, 3(3), 219-221.

Soothill, E., & Fairhurst, A. (1978). The new field guide to fungi. Michael Joseph Ltd..

Teoh YP, Mat D. 2010. Screening of selected fungi for biological control agent potential towards wood-degrading fungi of Malaysia. Malaysian Journal of Pharmaceutical Sciences 1: 1.

Yamaç, M., & Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates. Pharmaceutical biology, 44(9), 660-667..

Zjawiony, J. K. (2004). Biologically active compounds from Aphyllophorales (polypore) fungi. Journal of natural products, 67(2), 300-310.




How to Cite

ASHRAF, I., ALI, M., ASIF, M., ANWAR, W., AKHTAR, A., ALI, H., BUKHARI, N., ALI, Q., & SHAFIQ, M. (2023). UNVEILING ANTIFUNGAL PROTEINS FROM TRAMETES VERSICOLOR AS BIO-PESTICIDE TO INHIBIT ALTERNARIA SOLANI. Biological and Clinical Sciences Research Journal, 2023(1), 185. https://doi.org/10.54112/bcsrj.v2023i1.185

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 > >>