ANTIBACTERIAL, ANTIOXIDANT ACTIVITIES AND ASSOCIATION AMONG PLANT GROWTH RELATED TRAITS OF LEPIDIUM DRABA

Q Ali; R Khalil; M Nadeem; MM Azhar; MM Hafeez; A Malik

doi:10.54112/bcsrj.v2020i1.11

Authors

Q Ali Institute of Biotechnology and Molecular Biology, The University of Lahore, Lahore
R Khalil Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
M Nadeem Wheat Research Institute, Ayub Agriculture Research Institute Faisalabad, Pakistan
MM Azhar Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
MM Hafeez Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
A Malik Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2020i1.11

Keywords:

Lepidium draba, antibacterial, antioxidant, phenols, ethanol, n-hexan, plant extract

Abstract

The Lepidium draba or white tope also known as hoary cress belongs to family Brassicaceae, has been known as one of the perennial herbs which grow normally from seeds and also produced horizontal long creeping roots. It normally grows as a weed plant in farm fields. The present study was conducted to find out the antibacterial and antioxidant activities along with the plant traits of Lepidium draba. The shoot, root and inflorescence extracts were taken through using ethanol, water and n-hexan as extraction solvents. It was found from our studies that the plant extracts were heaving alkaloids, saponins, flavonoids, coumarins, anthocyans, quinons, steroids, sterols and terpenoids as potential phenolic compounds. The bacterial strains including Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus were cultured to access the antibacterial activities of plant extracts. The higher antibacterial activity was reported from n-hexan extract which indicate that the n-hexan extract may be used for extracting phytochemicals to be used as potential antibacterial biomedicines. The water extract showed higher antioxidant activities as compared with ethanol and n-hexan extracts which indicated that the use of water extract may be as a useful antioxidant under stress conditions, may also have anticancer activities. The lower coefficient of variation was recorded for all of the studied traits which indicated the consistency of results and reliability of selecting plants from various locations. There was significant and positive correlation among plant height with inflorescence weight, leaf area and leaf length, the inflorescence weight showed positive correlation with plant height, lea length and leaf area. There was higher contribution of plant height while leaf area contributed lower and negatively towards fresh plant weight. We have concluded from our study that the Lepidium draba plant extract showed antibacterial and antioxidant activities through using ethanol, water and n-hexan as extraction solvents. The significant correlation and regression associations indicated that the plants can tolerate harsh environmental conditions.

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References

Agarwal, J., and Verma, D. (2011). Antioxidative activity and flavonoid composition from Lepidium sativum. Nat. Sci 9, 21-25.

Al-Marzoqi, A. H., Al-Khafaji, K. R., and Kadhim, A. (2015). Influence of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Cardaria draba (Lepidium draba L.) on Human Pathogenic Bacteria. World Journal of Pharmaceutical Research 4, 456-460.

Ali, Q., Ahsan, M., Ali, F., Aslam, M., Khan, N. H., Munzoor, M., Mustafa, H. S. B., and Muhammad, S. (2013). Heritability, heterosis and heterobeltiosis studies for morphological traits of maize (Zea mays L.) seedlings. Advancements in Life sciences 1.

Ali, Q., Ahsan, M., Kanwal, N., Ali, F., Ali, A., Ahmed, W., Ishfaq, M., and Saleem, M. (2016). Screening for drought tolerance: comparison of maize hybrids under water deficit condition. Advancements in Life Sciences 3, 51-58.

Ali, Q., Ali, A., Ahsan, M., Nasir, I. A., Abbas, H. G., and Ashraf, M. A. (2014). Line× Tester analysis for morpho-physiological traits of Zea mays L seedlings. Advancements in Life sciences 1, 242-253.

Anwer, S., Ali, Q., Ali, M., Khalid, H., Ahmad, A., Ali, A., Shafiq, M., Haider, M., Nasir, I., and Husnain, T. (2015). Assessment of association among various morphological traits of Euphorbia granulata, Euphorbia hirta, Fumaria indica and Parthenium hysterophorus. Nature and Science 13, 47-51.

Benakashani, F., Allafchian, A., and Jalali, S. A. H. (2017). Green synthesis, characterization and antibacterial activity of silver nanoparticles from root extract of Lepidium draba weed. Green chemistry letters and reviews 10, 324-330.

Chyad, A. (2017). Evaluation of anticancer, analgesic and anti-inflammatory activities of the ethanolic extract of Lepidium draba Linn. leaves. Adv. Anim. Vet. Sci 5, 7-13.

Fursa, N., Litvinenko, V., and KRIVENCUK, P. (1970). Flavonol glycosides in Lepidium latifolium and L. draba. Rastitel'nye Resursy 6, 567-71.

Harborne, A. (1998). "Phytochemical methods a guide to modern techniques of plant analysis," springer science & business media.

Mahmood, S., Ali, Q., Ahmad, A., Ali, A., Babar, Y., Samiullah, T. R., Azam, S., Saeed, A., Qurat-ul-Ain Sajid, F. A., and Anjum, A. (2015). Estimation of correlation among various morphological traits of Carthamus oxycantha, Cirsium arvense, Cleome viscose and Convolvulus arvensis. World Rural Observations 7, 42-46.

Miri, A., Rad, J. S., Rad, M. S., and Silva, J. (2013). Allelopathic activity of medical plant, Cardaria draba (Lepidium draba L.). Annals of Biological Research 4, 76-79.

Mobeen, A., Qurban, A., Sadia, A., Harrem, K., Ali, A., Arfan, A., Muhammad, S., Muhammad, S., Idrees, A., and Tayyab, H. (2015). Estimation of Correlation among various morphological traits of Coronopus didymus, Euphorbia helioscopia, Cyperus difformis and Aristida adscensionis. NY Sci J 8, 47.52.

Ouissem, B. S., Sabrina, B., Lotfi, B., Khellaf, R., Chawki, B., Ibrahim, D., Samir, B., and Fadila, B. (2018). HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract. Journal of Biologically Active Products from Nature 8, 265-271.

Rad, J. S., Rad, M. S., and da Silva, J. A. T. (2014). Evaluation of allelopathic effects of methanolic extracts from Salicornia herbacea seed and leaves on germination and seedling growth in vitro of two medicinal plants and two weeds. Environmental and Experimental Biology 12, 83-87.

Reed, C. F. (1970). "Selected weeds of the United States," US Department of Agriculture.

Roughani, A., Miri, S. M., Hassandokht, M. R., Moradi, P., and Abdossi, V. (2018). Morphological variation of some Lepidium draba and L. latifolium populations. Taiwania 63.

Sadia, A., Qurban, A., Mobeen, A., Harrem, K., Ali, A., Arfan, A., Muhammad, S., Muhammad, S., Idrees, A., and Tayyab, H. (2015). Assessment of association among various morphological traits of Euphorbia granulata, Euphorbia hirta, Fumaria indica and Parthenium hysterophorus. Nat Sci 13, 47-51.

Scurfield, G. (1962). Cardaria Draba (L.) Desv. Journal of Ecology 50, 489-499.

Sharifi-Rad, J., Hoseini-Alfatemi, S. M., Sharifi-Rad, M., da Silva, J. A. T., Rokni, M., and Sharifi-Rad, M. (2015). Evaluation of biological activity and phenolic compounds of Cardaria draba (L.) extracts. J. Biol. Today's World 4, 180-189.

Trease, G., and Evans, W. (1983). Textbook of Pharmacognosy. (Balliere. Tindall, London, 57-59.

Usman, S., Qurban, A., Naveed, M., and Muhammad, S. (2012). Correlation analysis of seed yield and its components in chickpea (Cicer arietinum L.) genotypes. International Journal for Agro Veterinary and Medical Sciences (IJAVMS) 6, 269-276.

Vasilakoglou, I. B., Dhima, K., and Eleftherohorinos, I. (2006). Hoary cress (Cardaria draba) control in winter wheat with postemergence herbicides. Weed technology 20, 585-592.

Zameer, M., Munawar, S., Tabassum, B., Ali, Q., Shahid, N., Saadat, H. B., and Sana, S. (2015). Appraisal of various floral species biodiversity from Iskandarabad, Pakistan. Life Sci J 12, 77-87.