ANTIBIOGRAM ASSAY OF E.COLI ISOLATES IN PATIENTS WITH URINARY TRACT INFECTION

Authors

  • S AHMAD Centre for Biotechnology and Microbiology, University of Swat, Pakistan
  • H KHAN Department of Genomics, Phenomics, and Bioinformatics, North Dakota State University USA
  • F HANAN Saidu Medical college/Saidu Group of Teaching Hospital Swat, Pakistan
  • SU KHAN Hazara University Mansehra, Pakistan
  • MS FAISAL Department of Zoology, Parasitology Laboratory, Faculty of Biological Sciences, Quaid-I-Azam University Islamabad, Pakistan
  • I ULHAQ Department of Zoology, Parasitology Laboratory, Faculty of Biological Sciences, Quaid-I-Azam University Islamabad, Pakistan
  • M SHAH Centre for Biotechnology and Microbiology, University of Swat, Pakistan
  • L ALI Centre for Biotechnology and Microbiology, University of Swat, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2024i1.1126

Keywords:

Antibiotic Resistance, Escherichia coli, Susceptibility, Drug, Urinary Tract Infections, Urine Microbiology

Abstract

Screening of prevalent bacterial pathogens for antibiotic susceptibility profiles in different regions is necessary.  E. coli and other pathogens are so common in our environment that it is essential to monitor trends in antibiotic susceptibility regularly. Objective: The aim of the study was to find out the antibiotic susceptibility pattern of E.coli isolates in patients with urinary tract infection. Methods: This study was conducted at the Saidu Group of Teaching Hospital Swat after taking permission from the ethical committee of the institute.  We collected a total of 900 urine samples from males and females of all ages for culture and sensitivity.  The Kirby-Bauer disc diffusion method was used to assess the antibiogram on Mueller-Hinton agar. The plates were kept in incubator at 37°C for the 48 hours, and the inhibition zone was measured. The antibiogram was performed by Kirby-Bauer disc diffusion method applying various antibiotics. The bacterial strains were classified in to three majors groups on the basis of antibiotic susceptibility pattern resistant (R) sensitive (S), and intermediate (I). Data was statistically   analyzed presented in the tables and figures. Results: For culture and sensitivity testing, a total of 900 urine samples from patients with suspected UTIs were obtained from the several departments' inpatient and outpatient to the microbiological lab.  Substantial bacteriuria was noted in 250 (27.7%).  The most prevalent bacteria isolated was E.coli (55%) and its antibiogram was carried out. Ampicillin had the highest proportion of resistance (96.1%), followed by Ceftriaxone (91%), Moxifloxacin (87.1%), and Ceftazidime (75.5%).  Whereas it was sensitive to Fosfomycin (94.2%), followed by Sulzone ( 84.3%), Imepinem (84.3%) and Amikacin (78%). Conclusion: It was concluded from the current study that the most prevalent bacteria causing urinary tract infection was E.coli, and this bacteria showed remarkable resistance against Ampicillin   Ceftriaxone, Moxifloxacin, and Ceftazidime. The most used bacterium antibiotics for therapy were Fosfomycin, Imepinem and Amikacin. Therefore, antibiotic susceptibility should be considered before therapy.

Downloads

Download data is not yet available.

References

Kulkarni R, Dhakal BK, Slechta ES, Kurtz Z, Mulvey MA, Thanassi DG. Roles of putative type II secretion and type IV pilus systems in the virulence of uropathogenic Escherichia coli. PLoS One. 2009;4(3):e4752.

Karlowsky JA, Jones ME, Draghi DC, Thornsberry C, Sahm DF, Volturo GA. Prevalence and antimicrobial susceptibilities of bacteria isolated from blood cultures of hospitalized patients in the United States in 2002. Annals of clinical microbiology and antimicrobials. 2004;3:1-8.

Chen Y-H, Hsueh P-R, Badal RE, Hawser SP, Hoban DJ, Bouchillon SK, et al. Antimicrobial susceptibility profiles of aerobic and facultative Gram-negative bacilli isolated from patients with intra-abdominal infections in the Asia-Pacific region according to currently established susceptibility interpretive criteria. Journal of Infection. 2011;62(4):280-91.

Hawser SP, Badal RE, Bouchillon SK, Hoban DJ, Group SIW. Antibiotic susceptibility of intra-abdominal infection isolates from Indian hospitals during 2008. Journal of medical microbiology. 2010;59(9):1050-4.

Russo TA, Johnson JR. Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC. The Journal of infectious diseases. 2000;181(5):1753-4.

Hassan HS. Sensitivity of Salmonella and Shigella to antibiotics and chemotherapeutic agents in Sudan. The Journal of Tropical Medicine and Hygiene. 1985;88(4):243-7.

Aibinu IE, Adenipekun EO, Nwaka D, Adelowotan AO, Ajekigbe A, Adeyemi O, et al. Emergence of cross-resistance to fluoroquinolones in gram-negative isolates from cancer infections in a tertiary hospital in Nigeria. J Am Sci. 2008;4:14-20.

Omigie O, Enweani I, Ohenhen R, Umolu I, BenEdo-Osagie O. Bacteriological survey of wound infections in Benin City, Nigeria. Nig Ann Nat Sci. 2006;6:8-11.

Tanvir R, Hafeez R, Hasnain S. Prevalence of multiple drug resistant Escherichia coli in patients of urinary tract infection registering at a diagnostic laboratory in Lahore Pakistan. Pak J Zool. 2012;44(3):707-12.

Ko M-C, Liu C-K, Woung L-C, Lee W-K, Jeng H-S, Lu S-H, et al. Species and antimicrobial resistance of uropathogens isolated from patients with urinary catheter. The Tohoku journal of experimental medicine. 2008;214(4):311-9.

Munoz-Dávila M, Roig M, Yagüe G, Blázquez A, Salvador C, Segovia M. Comparative evaluation of Vitek 2 identification and susceptibility testing of urinary tract pathogens directly and isolated from chromogenic media. European journal of clinical microbiology & infectious diseases. 2013;32:773-80.

Wayne P. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2011.

Thattil SJ, Santhosh S. Prevalence of UTI in different age groups in a tertiary care hospital and their antibiogram. International journal of contemporary medical research. 2018;5(1):3-6.

Gupta S, Kapur S, Padmavathi D. Comparative prevalence of antimicrobial resistance in community-acquired urinary tract infection cases from representative States of northern and southern India. Journal of clinical and diagnostic research: JCDR. 2014;8(9):DC09.

Malik S, Rana JS, Nehra K. Prevalence and antibiotic susceptibility pattern of uropathogenic Escherichia coli strains in Sonipat region of Haryana in India. Biomedical and Biotechnology Research Journal (BBRJ). 2021;5(1):80-7.

Nijssen S, Florijn A, Bonten M, Schmitz F, Verhoef J, Fluit A. Beta-lactam susceptibilities and prevalence of ESBL-producing isolates among more than 5000 European Enterobacteriaceae isolates. International journal of antimicrobial agents. 2004;24(6):585-91.

Niranjan V, Malini A. Antimicrobial resistance pattern in Escherichia coli causing urinary tract infection among inpatients. Indian Journal of Medical Research. 2014;139(6):945-8.

Aziz Q, ALI Z, IZHAR M, Shah VH. Antimicrobial resistance;: comparison of Escherichia coli in different areas of lahore. The Professional Medical Journal. 2012;19(03):276-80.

Mavroidi A, Miriagou V, Liakopoulos A, Tzelepi Ε, Stefos A, Dalekos GN, et al. Ciprofloxacin-resistant Escherichia coli in Central Greece: mechanisms of resistance and molecular identification. BMC infectious diseases. 2012;12:1-7.

Mangaiarkkarasi A, Ivan E, Gopal R. Antimicrobial susceptibility patterns of clinical isolates of gram-negative pathogens from a teaching hospital, Pondicherry. 2013.

Sharma S, Gupta A, Arora A. Cefepime tazobactam: a new β lactam/β lactamase inhibitor combination against ESBL producing gram negative bacilli. 2012.

Downloads

Published

2024-09-20

How to Cite

AHMAD , S., KHAN, H., HANAN, F., KHAN, S., FAISAL, M., ULHAQ, I., SHAH, M., & ALI, L. (2024). ANTIBIOGRAM ASSAY OF E.COLI ISOLATES IN PATIENTS WITH URINARY TRACT INFECTION. Biological and Clinical Sciences Research Journal, 2024(1), 1126. https://doi.org/10.54112/bcsrj.v2024i1.1126

Most read articles by the same author(s)

1 2 3 > >>