EVALUATION THE HEPATOPROTECTIVE EFFECT OF QUERCETIN AGAINST ZINC OXIDE NANOPARTICLES INDUCED TOXICITY IN MOUSE MODEL

R ANJUM; H MAQSOOD; A ANWAR; S HUSSAIN; K ALEEM; S MOHSIN; S ASLAM; S KANWAL; A AJMAL; T AHMED; M EHTSHAM; M HAMID

doi:10.54112/bcsrj.v2023i1.246

Authors

R ANJUM Department of Zoology, Government College University, Lahore, Pakistan
H MAQSOOD Faisalabad Medical University, Allied Hospital Faisalabad, Pakistan
A ANWAR Pharmacology, Punjab Medical College Faisalabad, Pakistan
S HUSSAIN Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan
K ALEEM Department of Zoology, Riphah International University, Faisalabad Campus, Pakistan
S MOHSIN Department of Zoology, Government College University, Lahore, Pakistan
S ASLAM Department of Zoology, Riphah International University, Faisalabad Campus, Pakistan
S KANWAL Department of Zoology, Government College University, Lahore, Pakistan
A AJMAL Department of Zoology, Government College University, Lahore, Pakistan
T AHMED School of Biochemistry & Biotechnology, University of the Punjab, Lahore, Pakistan
M EHTSHAM Department of Emergency Medicine,Tehsil Headquarters Hospital, Chak Jhumra, Faisalabad, Pakistan
M HAMID Registrar, Family Physician, King Saud University Medical City, Riyadh, Saudi Arabia

DOI:

https://doi.org/10.54112/bcsrj.v2023i1.246

Keywords:

Quercetin, zinc oxide, nanoparticles, liver, mouse model

Abstract

Quercetin is a well-known flavonoid that resents in fruits and vegetables. Zinc oxide nanoparticles (ZnO-NPs) are widely used for packing and cosmetics. This study was conducted to determine the effect of Quercetin on the liver by ZnO-NPs toxicity in a mouse model. Thirty-six Swiss albino mice were divided into two groups, i.e. control group and the experimental group. The control group was administered saline for 28 days orally. The experimental group is further divided into three groups. To the first one, only ZnO-NPs (30 mg/kg, orally) were given for 28 days. To the second group, only quercetin (100 mg/kg, orally) was given for 28 consecutive days. To the third, a combination of quercetin and ZnO-NPs was given for alternate 28 days through the oral route. Animals were sacrificed after 14 and 28 days of dosing. Mouse liver was fixed in the 10% formalin to preserve organ integrity. Histopathological analysis was done to observe the structure of liver in treated mice. The ZnO-NPs group shows the central vein full of infiltrations, sinusoidal spaces congestions and some the necrosis of hepatocytes was observed. The quercetin group shows a normal central vein with no infiltrations. The animals that received both quercetin and ZnO-NPs showed infiltration in central vein and necrosis but less than that of only ZnO-NPs given group. Full recovery in the liver structure was observed in the quercetin+ZnO-NPs group after 28 days of treatment. This study showed the hepatoprotective effect of quercetin because it reduces the toxicity produced by ZnO-NPs.

Downloads

Download data is not yet available.

References

Ames, B. N., McCann, J. and Yamasaki, E. 1975. Methods for detecting carcinogens and mutagens with the Salmonella mammalian-microsome mutagenicity test. Mutation Research, 31(6): 347-364.

Amin, A. and Hamza, A. A. 2005. Oxidative stress mediates drug-induced hepatotoxicity in rats: a possible role of DNA fragmentation. Toxicology, 208(3): 367-375.

Bengmark, S., Mesa, M. D., & Gil, A. 2009. Plant-derived health-the effects of turmeric and curcuminoids. Nutricion hospitalaria, 24(3), 273-281.

Buzea, C., Pacheco, II and Robbie, K. 2007. Nanomaterials and nanoparticles: Sources and toxicity. Biointerphases, 2(4): 17-71.

Cabiscol, E., Tamarit, J., and Ros, J. 2000. Oxidative stress in bacterial and protein damage by reactive oxygen species. International Microbiology, 3(1): 3–8.

Choi, E. J., Chee, K. M., and Lee, B. H. 2003. Anti-and prooxidant effects of chronic quercetin administration in rats. European Journal of Pharmacology, 482(1), 281-285.

Gerloff, K., Albrecht, C., Boots, A., Förster, I. and Schins, R. P. F. 2009. Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells. Nanotoxicology, 5(4): 355–364.

Huang, Y. W., Wu, C. H., and Aronstam, R. S. 2010. Toxicity of transition metal oxide nanoparticles: recent insights from in vitro studies. Materials, 3(10), 4842-4859.

Hussain, N., Jaitley, V. and Florence, A. T. 2009. Designing and surface modification of zinc oxide nanoparticles for biomedical applications. Food and Chemical Toxicology, 49(9), 2107-2115.

Li, S., Tan, H. Y., Wang, N., Zhang, Z. J., Lao, L., Wong, C. W. and Feng, Y. 2015. The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Sciences, 16(11), 26087-26124.

Liu, H., Ma, L., Zhao, J., Liu, J., Yan, J., Ruan, J., and Hong, F. 2009. Biochemical toxicity of nano-anatase TiO2 particles in mice. Biological Trace Element Research, 129(1-3): 170-180.

Ma, L., Zhao, J., Wang, J., Liu, J., Duan, Y. and Liu, H. 2010. The acute liver injury in mice caused by nano-anatase TiO2. Nanoscale Research Letter, 4(11): 1275-1285.

Ma, L., Zhao, J., Wang, J., Liu, J., Duan, Y., Liu, H. and Hong, F. 2009. The acute liver injury in mice caused by nano-anatase TiO 2. Nanoscale Research Letters, 4(11): 1275.

Mark, R., Wick, M. D., Nancy, C., William, K., Mills, H. T. and Brix, M. D. 2013. Tissue Procurement, Processing, and Staining Techniques. Diagnostic Histochemistry. 1-27. Cambridge University Press 978-0-521-87410-6 .

Rasmussen, J. W., Martinez, E., Louka, P. and Wingett, D. G. 2010. Zinc oxide nanoparticles for selective destruction of tumor cells and potential for drug delivery applications. Expert Opinion on Drug Delivery, 7(9): 1063-1077.

Schilling, K., Bradford, B. Castelli, D., Dufour, E. J. F. Nash, J. F., Pape, W., Schulte, S., Tooley, I., van den Bosch, J. and Schellauf, F. 2010. Human safety review of nano titanium dioxide and zinc oxide. Photochemical and Photobiological Sciences, 9(1): 495–509.

Sha, B., Gao, W., Wang, S., Gou, X., Li, W. and Liang, X. 2014. Oxidative stress increased hepatotoxicity induced by nano-titanium dioxide in BRL-3A cells and Sprague-Dawley rats. Journal of Applied Toxicology, 34(4): 345-356.

Sharma, V., Singh, P., Pandey, A. K. and Dhawan, A. 2012. Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 745(1), 84-91.

Sycheva, L. P., Zhurkov, V. S., Iurchenko, V. V., Daugel-Dauge, N. O., Kovalenko, M. A., Krivtsova, E. K. and Durnev, A. D. 2011. Investigation of genotoxic and cytotoxic effects of micro-and nanosized titanium dioxide in six organs of mice in vivo. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 726(1), 8-14.

Wang, H., Wick, R. L. and Xing, B. 2009. Toxicity of nanoparticulate and bulk ZnO, Al2O3 and TiO2 to the nematode Caenorhabditis elegans. Environmental Pollutants, 157(1): 1171–1177.

Xia, T., Kovochich, M., Liong, M., Mädler. L,, Gilbert, B. and Shi, H. 2006. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nanoparticle, 2(10): 2121-2134.

Yoshio, K., Sippel, H. W., Penttilä, K. E., and Lindros, K. O. 1987. Acinar distribution of glutathione-dependent detoxifying enzymes: Low glutathione peroxidase activity in perivenous hepatocytes. Biochemical Pharmacology, 36(12), 2003-2006.

Zhang, X. D., Wu, H. Y., Wu, D., Wang, Y. Y., Chang, J. H. and Zhai, Z. B. 2010. Toxicological effects of gold nanoparticles in vivo by different administration routes. International Journal of Nanomedicine, 5(1): 771-81.