RELATIONSHIP BETWEEN HIGH-TEMPERATURE STRESS AND KEY PHYSIO-CHEMICAL, REACTIVE OXYGEN SPECIES AND ANTIOXIDANTS IN SPRING MAIZE HYBRIDS UNDER SEMI-ARID CONDITIONS
Keywords:High temperature, biplot analysis, climate change, photosynthesis, antioxidants
High-temperature stress is one of the hurdles to achieving self-sufficiency and sustainability in maize production globally. The current experimental study was executed to identify the best suitable maize hybrids for heat-prone areas based on their performance. During spring 2020 & 2021, hybrids were sown under two stress conditions (a) control sowing and (b) late sowing. Kernel yield and related characteristics varied significantly among maize hybrids across both situations (P<0.05). Under High-temperature stress, correlation analysis uncovered a positive relationship between kernel yield and chlorophyll a (r = 0.77**, 0.54**), chlorophyll b (r = 0.72**, 0.66**), net photosynthetic rate (r = 0.71**, 0.67**), proline contents (r = 0.59*, 0.54**), hydrogen peroxide (r = 0.54*, 0.17NS), thousand kernel weight (r = 0.71*, 0.38*). Principal component and biplots analysis unveiled that the first four principal components accountable for 78.0% of the total variability among hybrids, with days to 50% silking, plant height, number of kernels per ear, kernel yield, net photosynthetic rate, hydrogen peroxide, malondialdehyde, and catalase as the primary sources of variation. Agglomerative Hierarchical Clustering (AHC) categorizes indigenous and multinational maize hybrids into three classes under stress treatments. The cluster analysis further revealed that indigenous hybrids, particularly YH-5395, YH-5482 and YH-5427 were the most heat tolerant and productive hybrids while YH-5404, P-1543 and JPL-1908 were among the most heat susceptible ones. Consequently, these hybrids are recommended for widespread cultivation, particularly in regions prone to high temperatures.
Al-Naggar, A. M. M., Shafik, M. M., and Musa, R. Y. M. (2020). Genetic Diversity Based on Morphological Traits of 19 Maize Genotypes Using Principal Component Analysis and GT Biplot. Annual Research & Review in Biology 35(2), 68-85
Ashraf, M. and Harris, P.J. (2013) Photosynthesis under stressful environments: an overview. Photosynthetica 51, 163-190.
Asseng, S., Ewert, F., Rosenzweig, C., Jones, J.W., Hatfield, J.L., Ruane, A.C., et al. (2013) Uncertainty in simulating wheat yields under climate change. Nature Climate Change, 3: 827
Ben-Asher, J., Garcia, A., and Hoogenboom, G. (2008). Effect of high temperature on photosynthesis and transpiration of sweet corn (Zea mays L. var. rugosa) . Photosynthetica, 46(4), 595-603.
Bhatti, M. H., Yousaf, M. I., Ghani, A., Arshad, M., Shehzad, A., Mumtaz, A., Khalid, M.U., Khalid, M.Z., Mushtaq, M.Z., and Shah, S.A.S. (2020). Assessment of genetic variability and traits association in upland cotton (Gossypium hirsutum L.). International Journal of Botany Studies 5(2), 148-151.
Chance, B., and Maehly, A. C. (1955). Assays of catalases and peroxidases. Methods Enzymol 2, 764–775.
Efeoglu, B., Ekmekçi, Y., and Çiçek, N. (2009). Physiological responses of three maize cultivars to drought stress and recovery. South African Journal of Botany 75, 34–42.
ESP (2021). Economic Survey of Pakistan. 2020-21, Ministry of Finance, Govt. of Pakistan. Available online at Accessed on April 3, 2022. https://www.finance. gov.pk/survey/chapters_21/02-Agriculture.pdf
Ghani, A., Yousaf, M.I., Arshad, M., Hussain, K., Hussain, S., Hussain, D., Hussain, A., and Shehzad, A. (2020). YH-5427: A highly productive, heat tolerant, stalk rot and lodging resistance, yellow maize hybrid of Punjab, Pakistan. International Journal of Biology and Biotechnology 17 (3), 561-570.
Hall, M. L. (2002). Heat stress effects on growth, yield, and quality of maize. Agronomy Journal 94(5), 1073-1081.
Heath, R. L., and Packer, L. (1968). Photoperoxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics 125, 189–198.
Hussain, H. A., Men, S., Hussain, S., Chen, Y., Ali, S., Zhang, S., Zhang, K., Li, Y., Xu, Q., Liao, C., and Wang, L. (2019). Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Scientific reports, 9(1), 3890.
Khajeh-Hosseini, M., Teixeira da Silva, J. A., and Siddique, K. H. M. (2016). Heat stress in crop plants: a review on morphological characteristics and possible mechanisms of tolerance. Frontiers in Plant Science 7, 930.
Khalid, M. U., Akhtar, N., Arshad, M., and Yousaf, M.I. (2020). Characterization of maize inbred lines for grain yield and related traits under heat stress conditions. International Journal of Biology and Biotechnology 17(2), 367-375.
Lambert, R. J., Mansfield, B.D., and Mumm, R.H. (2014). Effect of leaf area on maize productivity. Maydica 59(1), 58-63.
Liu, X. J., Li, Y. X., Wang, G. L., and Zhang, G. S. (2010). Heat stress-induced alterations in the growth, photosynthesis and antioxidant metabolism of maize leaves. Physiologia Plantarum 139(4), 537-548.
Lobell, D. B., Bänziger, M., Magorokosho, C., and Vivek, B. (2011). Nonlinear heat effects on African maize as evidenced by historical yield trials. Nature Climate Change 1, 42–45.
Sabagh, A. E., Hossain, A., Iqbal, M. A., Barutçular, C., Islam, M. S., Çiğ, F., Erman, M., Sytar, O., Brestic, M., Wasaya, A., Jabeen, T., and Saneoka, H. (2020). Maize adaptability to heat stress under changing climate. In Plant stress physiology. IntechOpen.
Saeed, M., Mumtaz, A., Hussain, D., Arshad, M., Yousaf, M. I., and Ahmad, M. S. (2018). Multivariate analysis-based evaluation of maize genotypes under high temperature stress. I3 Biodiversity, 1.
Schmidhuber, J., & Tubiello, F. N. (2007). Global food security under climate change. Proceedings of the National Academy of Sciences 104(50), 19703-19708.
Sergiev, I., Alexieva, V., and Karanov, E. (1997). Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Proceedings of the Bulgarian Academy of Sciences 51, 121–124.
Shehzad, A., Yousaf, M.I., Ghani, A., Hussain, K., Hussain, S., and Arshad, M. (2019). Genetic analysis and combining ability studies for morpho-phenological and grain yield traits in spring maize (Zea mays L.). International Journal of Biology and Biotechnology 16(4), 925-931.
Singh, B. K., Jain, S., and Srivastava, G. C. (2013). Heat stress in crop plants: a review on morphological, physiological, biochemical and molecular aspects of heat stress response and tolerance. Plant Physiology and Biochemistry 71, 2-30.
Sneath, P. H. A. and Sokal, R. R. (1973). Numerical Taxonomy: The Principles and practice of numerical classification. Free-Man WF and Co, San Francisco, USA.
Steel, R. G. D., Torrie, J. H. and Dickey, D. A. (1997). Principles and Procedures of Statistics: A Biometrical Approach, 3rd Ed. McGraw Hill Book Co., New York.
Tiwari, Y. K., and Yadav, S. K. (2019). High temperature stress tolerance in maize (Zea mays L.): Physiological and molecular mechanisms. Journal of Plant Biology 62, 93-102.
USDA. 2022. United State Department of Agriculture: World Agricultural Production. United States Department of Agriculture, Circular series. WAP. 08-22. United State.
Waqas, M. A., Wang, X., Zafar, S. A., Noor, M. A., Hussain, H. A., Azher Nawaz, M., and Farooq, M. (2021). Thermal stresses in maize: effects and management strategies. Plants 10(2), 293.
Yousaf, M. I., Hussain, K., Hussain, S., Shahzad, S., Ghani, A., Arshad, M. Mumtaz, A. and Akhtar, N. (2017). Morphometric and phenological characterization of maize (Zea mays L.) germplasm under heat stress. International Journal of Biology and Biotechnology 14(2), 271-278.
Yousaf, M. I., Hussain, K., Hussain, S., Ghani, A., Arshad, M., Mumtaz, A., and Hameed, R.A. (2018). Characterization of indigenous and exotic maize hybrids for grain yield and quality traits under heat stress. International Journal of Agriculture and Biology 20(2), 333-337.
Yousaf, M.I., Bhatti, M.H., Maqbool, M.A., Ghani, A., Akram, M., Ibrar, I. Khan, A., Khan, R.A.H., Kohli, S.A., Siddiq, M.A.B., Khalid, M.U. (2021)Heat stress induced responses in local and multinational maize hybrids for morphophysiological and kernel quality traits. Paistan Journal of Agricultural Sciecnces 58: 1511-1521.
Yousaf, M. I., Hussain, K., Hussain, S., Ghani, A., Bhatti, M. H., Mumtaz, A., Khalid, M.U., Mehboob, A., Murtaza, G., and Akram, M. (2022). Characterization of maize (Zea Mays L.) hybrids for physiological attributes and grain quality traits under heat stress. Iranian Journal of Plant Physiology 12(2), 4075-4087.
Yousaf, M.I., Riaz, M.W., Jiang, Y., Yasir, M., Aslam, M.Z., Hussain, S., Shah, S.A.S., Shehzad, A., Riasat, G., Manzoor, M.A., and Akhtar, I. (2022). Concurrent Effects of Drought and Heat Stresses on Physio-Chemical Attributes, Antioxidant Status and Kernel Quality Traits in Maize (Zea mays L.) Hybrids. Frontiers in Plant Science 13. 898823.
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Copyright (c) 2023 A GHANI, MI YOUSAF, K HUSSAIN, S HUSSAIN, A RAZAQ, N AKHTAR, I IBRAR, N KAMAL, B ALI, AM KHAN, SWH SHAH, S KHANUM, RM HASSAN
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