• R Ejaz Department of Biotechnology, Kinnaird College for Women, 93 Jail Road, Lahore, Punjab, Pakistan
  • S Malik 2Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  • M Ahmad Department of Biotechnology, Kinnaird College for Women, 93 Jail Road, Lahore, Punjab, Pakistan
  • H ALi College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China
  • S Choudhry Department of Biotechnology, Kinnaird College for Women, 93 Jail Road, Lahore, Punjab, Pakistan



menthol, Mentha piperita, anti-bioflim, Pseudomonas aeruginosa, inhibition


Menthol, a bioactive compound of Mentha piperita (mint) with antibacterial properties was purified by column chromatography to determine its anti-biofilm potential. After phytochemical analysis, TLC was carried out using n-hexane: ethyl acetate: methanol: water (2:2:2:1) as the solvent system for ethanolic extract of mint. TLC achieved the maximum separation of mint constituents with Rf value of 0.68. A purified menthol fraction was obtained after silica gel column chromatography using four different eluting solvents. The menthol obtained was then used to perform biofilm inhibition assay to establish its antibacterial potential. Percentage inhibition was highest for bacillus subtilis (79.4%), as opposed to Pseudomonas aeruginosa (33.6%) and the combination of both bacteria (20%). ELISA reader was used to measure absorbance at 450-620nm and 630 nm. Using 450-620nm filter the values for percentage inhibition lies between 48.6-95% for standard and crude menthol samples. Similarly, at 630nm the values of inhibition lie between 23.4-70.6%. This anti-biofilm property of menthol can be utilized in antibacterial drug formulations.


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How to Cite

Ejaz, R., Malik, S., Ahmad, M., ALi, H., & Choudhry, S. (2020). ANTI-BIOFILM POTENTIAL OF MENTHOL PURIFIED FROM MENTHA PIPERITA L. (MINT). Biological and Clinical Sciences Research Journal, 2020(1).



Original Research Articles