ANTIMICROBIAL POTENTIAL OF ETHANOLIC LEAF EXTRACTS OF PARKINSONIA ACULEATE USING RESPONSE SURFACE METHODOLOGY

NS YOUNAS; I ALI; H ASHRAF; NT BUKHARI

doi:10.54112/bcsrj.v2022i1.160

Authors

NS YOUNAS Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore Pakistan
I ALI Department of Life Sciences, School of Sciences, University of Management and Technology (UMT), Lahore, Pakistan
H ASHRAF Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore Pakistan
NT BUKHARI Microbiology and Clinical Laboratory Sciences, Women University Swabi, KPK, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2022i1.160

Keywords:

phytocompounds, Parkinsonia aculeata, Escherichia coli, flavonoids, alkaloids, tannins

Abstract

Plant extracts are commonly used to inhibit bacterial activities. In the current analysis, we aimed to study the antimicrobial activity of Parkinsonia aculeata. The plant powder was used to extract the phytocompounds present in the plant by standard method. Then this extract was used to inhibit the growth of different bacteria at different pH, temperate and concentration. Antimicrobial activity was noted under different conditions. These results showed that Parkinsonia aculeata has very important antibacterial compounds such as phenols, flavonoids, tannins, and alkaloids depicting promising antibacterial activity for various bacterial ailments but at a specific temperature, pH and concentration. Escherichia coli showed growth restriction at the optimal pH 5.0, temperature 35°C and concentration 5-10mg/dl. Salmonella typhi growth was inhibited at the optimal pH 5.0, temperature 35°C and concentration 9.4-10 mg/dl. Similarly, the Klebsiella pneumoniae offered the inhibition rate at the optimal pH 5-7, temperature 35.2°C and concentration 5.0-7.0mg/dl. All of these results were confirmed by contour plots by Response Surface Methodology. Hence, it is suggested from this research work that Parkinsonia aculeata, when used at proper pH, temperature and concentration, can be employed to treat various bacterial inflammations in the biological system.

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References

Alanis, A. J. (2005). Resistance to antibiotics: are we in the post-antibiotic era?. Archivesof Medical Research, 36(6), 697-705.

Ammer, M. R., Zaman, S., Khalid, M., Bilal, M., Erum, S., Huang, D., & Che, S. (2016).

Optimization of antibacterial activity of Eucalyptus tereticornis leaf extractsagainst Escherichia coli through response surface methodology. Journal ofRadiation Research and Applied Sciences, 9(4), 376-385.

Anandhi, D., Srinivasan, P. T., Kumar, G. P., & Jagatheesh, S. (2014). DNAfragmentation induced by the glycosides and flavonoids from C. coriaria.

International Journal of Current Microbiology and Applied Sciences, 3(12), 666-673.

Anandhi, D., Srinivasan, P. T., Praveen Kumar, G., & Jagatheesh, S. (2014). Influence offlavonoids and glycosides from Caesalpinia coriaria (Jacq) wild as bactericidalcompound. Internation Journal of Current Microbiology and Applied Sciences,3(4), 1043-51.

Ayob, M. K., & Tan, Y. N. (2013). Natural products-current and future promising sourceof novel drugs: A review on their antimicrobial mechanism of actions. Microbialpathogens and strategies for combating them: Science, technology and education.2:1196-1208.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluatingantimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79.

Bérdy, J. (2012). Thoughts and facts about antibiotics: where we are now and where weare heading. The Journal of antibiotics, 65(8), 385-395.

Borges, A., Ferreira, C., Saavedra, M. J., & Simões, M. (2013). Antibacterial activity andmode of action of ferulic and gallic acids against pathogenic bacteria. MicrobialDrug Resistance, 19(4), 256-265.

Chandra, H., Bishnoi, P., Yadav, A., Patni, B., Mishra, A. P., & Nautiyal, A. R. (2017).Antimicrobial resistance and the alternative resources with special emphasis onplant-based antimicrobials—A review. Plants, 6(2), 16.

Singh, A. (2008). Phytochemicals of Gentianaceae: A review of pharmacologicalproperties. International Journal of Pharmaceutical Sciences andNanotechnology, 1(1), 33-36.

Sledz, W., Los, E., Paczek, A., Rischka, J., Motyka, A., Zoledowska, S., ... & Lojkowska,E. (2015). Antibacterial activity of caffeine against plant pathogenic bacteria.Acta Biochimica Polonica, 62(3):605-612.

Tegtmeyer, N., Rohde, M., & Backert, S. (2012). Clinical presentations and pathogenicitymechanisms of bacterial foodborne infections. In Microbial Food Safety (pp. 13-31). Springer, New York, NY.

Thakur, D., Baishya, M., Sarma, B., Bora, T. C., & Saikia, R. (2008). Antimicrobialresistance in gram positive and gram-negative bacteria progress and challenges.Microbial Biotechnology. India: New India Publishing Agency, 349-75.

Ullah, N., & Khan, F. A. (2016). An introduction to natural products and phytochemicalswith special reference to its antimicrobial activity. Life Science Journal, 13(10),103-119.

Ultee, A., Kets, E. P. W., & Smid, E. J. (1999). Mechanisms of action of carvacrol on thefood-borne pathogen Bacillus cereus. Applied and Environmental Microbiology,65(10), 4606-4610.

Upadhyay, A., Upadhyaya, I., Kollanoor-Johny, A., & Venkitanarayanan, K. (2014).Combating pathogenic microorganisms using plant-derived antimicrobials: aminireview of the mechanistic basis. BioMed Research International, 15(3),12-17.

Vinoth, M. (2013). Antibacterial activity and the mode of action of alkaloid rich fractionfrom the leaves of Acalypha fruticosa. International Journal of EthnomedPharmacology Research 2013; 1 (1), 1, 6.

Wink, M., Ashour, M. L., & El-Readi, M. Z. (2012). Secondary metabolites from plantsinhibiting ABC transporters and reversing resistance of cancer cells and microbesto cytotoxic and antimicrobial agents. Frontiers in Microbiology, 3, 130.

Yoshida, K., Matsumoto, T., Tateda, K., Uchida, K., Tsujimoto, S., & Yamaguchi, K.(2000). Role of bacterial capsule in local and systemic inflammatory responses ofmice during pulmonary infection with Klebsiella pneumoniae. Journal of MedicalMicrobiology, 49(11), 1003-1010.