EFFECT OF DIFFERENT MEDIA COMPOSITIONS OF 2,4-D, DICAMBA, AND PICLORAM ON CALLUS INDUCTION IN WHEAT (TRITICUM AESTIVUM L.)

A ASHRAF; N AMHED; M SHAHID; T ZAHRA; Z ALI; A HASSAN; A AWAN; S BATOOL; MA RAZA; U IRFAN; Z MAQSOOD; MN KHALID; I AMJAD

doi:10.54112/bcsrj.v2022i1.159

Authors

A ASHRAF Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
N AMHED Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
M SHAHID Cotton Research Institute Multan, Pakistan
T ZAHRA Department of Botany, University of Agriculture Faisalabad, Pakistan
Z ALI Department of Agronomy, University of Agriculture, Faisalabad Pakistan
A HASSAN Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
A AWAN Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
S BATOOL Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
MA RAZA Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
U IRFAN Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
Z MAQSOOD Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
MN KHALID Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
I AMJAD Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2022i1.159

Keywords:

wheat, tissue culture, callus induction, picloram, dicamba

Abstract

Wheat is a major cereal crop grown worldwide. Genus Triticum contains many species, among which T. aestivumis has commonly grown also known as bread wheat. Its genome is hexaploid, containing 42 chromosomes (2n=6x=42). To improve the response of wheat cultivars against biotic and abiotic stresses, it is genetically engineered with different biotechnological tools. Tissue culture technology has been proved vital for improving crop species in different quality and yield-related traits. Being a monocotyledons plant, wheat shows recalcitration toward tissue culture. The response of wheat to regeneration can be improved by using different growth-promoting hormones. The proposed study's objective was to increase callus induction's efficiencyproposed study was to increase the efficiency of callus inductionproposed study's objective was to increase callus induction's efficiency using different treatment of growth regulators. MS media with varying concentrations of 4, 8, and 12 mg/L of dicamba, 2, 4-D, and picloram was used to check their effect on callus induction in common wheat cultivars Anaj-2017 and Akbar-2019. Media complemented with picloram at 8 mg/L was the supreme efficiency. About 511 mg and 420 mg of callus formation were observed at 8 mg/L for picloram succeeded by dicamba which was 340 mg and 350 mg at 12 mg/L, and then by 2, 4-D, which was 112 mg and 236 mg at 8 mg/L from matured embryos of Anaj-2017 and Akbar-2019 respectively.

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References

Alikina, О., Chernobrovkina, M., Dolgov, S., & Miroshnichenko, D. (2016). Tissue culture efficiency of wheat species with different genomic formulas. Crop breeding and applied biotechnology16, 307-314. DOI: https://doi.org/10.1590/1984-70332016v16n4a46

Boden, S. A., Cavanagh, C., Cullis, B. R., Ramm, K., Greenwood, J., Jean Finnegan, E., Trevaskis, B., & Swain, S. M. (2015). Ppd-1 is a key regulator of inflorescence architecture and paired spikelet development in wheat. Nature plants1, 1-6. DOI: https://doi.org/10.1038/nplants.2014.16

Breiman, A., & Graur, D. 1995. Wheat evolution. The Israel Journal of Plant Sciences43:85–98. DOI: https://doi.org/10.1080/07929978.1995.10676595

Briggle, L. (1967). Morphology of the wheat plant. Wheat and wheat improvement, 89-116.

Briggle, L. W., & Reitz, L. P. (1963). Classification of Triticum species and of wheat varieties grown in the United States. US Department of Agriculture.

Carman, J. (1988). Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments. Planta175, 417-424. DOI: https://doi.org/10.1007/BF00396349

Chawla, H. S. (2011). Introduction to plant biotechnology. CRC Press. DOI: https://doi.org/10.1201/9781315275369

Delporte, F., Mostade, O., & Jacquemin, J. (2001). Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant cell, tissue and organ culture67, 73-80. DOI: https://doi.org/10.1023/A:1011697316212

Dunwell, J. (1986). Pollen, ovule and embryo culture, as tools in plant breeding. Plant tissue culture and its agricultural applications, 375-404. DOI: https://doi.org/10.1016/B978-0-407-00921-9.50042-7

Hunsinger, H., & Schauz, K. (1987). The influence of dicamba on somatic embryogenesis and frequency of plant regeneration from cultured immature embryos of wheat (Triticum aestivum L.). Plant Breeding98, 119-123. DOI: https://doi.org/10.1111/j.1439-0523.1987.tb01103.x

Karsai, I., Bedo, Z., & Hayes, P. (1994). Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat. Plant cell, tissue and organ culture39, 49-53. DOI: https://doi.org/10.1007/BF00037591

Kippes, N., Zhu, J., Chen, A., Vanzetti, L., Lukaszewski, A., Nishida, H., Kato, K., Dvorak, J., & Dubcovsky, J. (2014). Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat. Molecular Genetics and Genomics289, 47-62. DOI: https://doi.org/10.1007/s00438-013-0788-y

Leach, L. J., Belfield, E. J., Jiang, C., Brown, C., Mithani, A., & Harberd, N. P. (2014). Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat. BMC genomics15, 1-19. DOI: https://doi.org/10.1186/1471-2164-15-276

MacKinnon, C., Gunderson, G., & Nabors, M. W. (1987). High efficiency plant regeneration by somatic embryogenesis from callus of mature embryo explants of bread wheat (Triticum aestivum) and grain sorghum (Sorghum bicolor). In vitro cellular & developmental biology23, 443-448. DOI: https://doi.org/10.1007/BF02623861

Majewsla, M., Sodkiewicz, W., & Sodiewicz, T. (2007). Callus induction and plant regeneration in propagation of wheat hybrids with introduced AM (Triticum monococcum) or R (Secale cereale) genome. Acta Biologica Cracoveinsia Series Botanica49, 39-44.

McHughen, A. (1983). Rapid regeneration of wheat in vitro. Annals of botany51, 851-853. DOI: https://doi.org/10.1093/oxfordjournals.aob.a086535

Murashige, T., & Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiolgical Planta. 15:473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Ozgen, M., Turet, M., Altinok, S., & SANCAK, C. (1998). Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant cell reports18. DOI: https://doi.org/10.1007/s002990050581

Papenfuss, J., & Carman, J. (1987). Enhanced regeneration from wheat callus cultures using dicamba and kinetin 1. Crop Science27, 588-593. DOI: https://doi.org/10.2135/cropsci1987.0011183X002700030035x

Shewry, P. R. (2009). Wheat. Journal of experimental botany60, 1537-1553. DOI: https://doi.org/10.1093/jxb/erp058

Zhou, M., & Lee, T. (1984). Selectivity of auxin for induction and growth of callus from excised embryo of spring and winter wheat. Canadian journal of botany62, 1393-1397. DOI: https://doi.org/10.1139/b84-189