Exploring the Link Between Biofilm Formation and Multidrug Resistance in Escherichia coli Isolated from Urinary Tract Infections in Sialkot, Pakistan

Kanwal  Shehzadi; Rooma  Adalat; Hafiz Abdul  Sattar; Hira  Ehsan; Saba  Manzoor; Uzma  Nisar; Sobia  Alyas; Sadaf  Ilyas

doi:10.54112/bcsrj.v6i8.1932

Authors

Kanwal Shehzadi Department of Zoology, Faculty of Science, University of Sialkot, Sialkot, Punjab, Pakistan
Rooma Adalat Department of Zoology, Faculty of Science, University of Sialkot, Sialkot, Punjab, Pakistan
Hafiz Abdul Sattar D. G. Khan Medical College, Dera Ghazi Khan, Punjab, Pakistan
Hira Ehsan Department of Zoology, Faculty of Science, University of Sialkot, Sialkot, Punjab, Pakistan
Saba Manzoor Department of Zoology, Faculty of Science, University of Sialkot, Sialkot, Punjab, Pakistan
Uzma Nisar School of Pharmacy, University of Management and Technology, Lahore, Pakistan
Sobia Alyas Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Punjab, Pakistan
Sadaf Ilyas Department of Zoology, Faculty of Science, University of Sialkot, Sialkot, Punjab, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v6i8.1932

Keywords:

Urinary Tract Infection, Biofilm, Multidrug Resistance, Infection, Sialkot

Abstract

Escherichia coli strains are among the key contributors to urinary tract infections (UTIs) around the world, presenting a significant health challenge globally. Objectives: This cross-sectional study aims to isolate multidrug-resistant (MDR) Escherichia coli strains from UTI patients in Sialkot, Pakistan, and to investigate their biofilm-forming ability, exploring the possible association between biofilm formation and antibiotic resistance. Methods: A total of 131 E. coli isolates were isolated and identified. Their ability to form biofilms was confirmed using the tube method, and their antibiotic susceptibility patterns were evaluated using the disc diffusion method. Fisher's exact test was applied to investigate the association between biofilm production and antibiotic resistance. Results: The findings revealed that 65.6% isolates (n = 88) were biofilm producers, while 34.4% (n = 43) were non-biofilm producers. Antibiotic sensitivity testing indicated high levels of resistance among isolates to several antibiotics, notably ceftriaxone (91.6%), cefoperazone (86.3%), ciprofloxacin (85.5%), cefotaxime (82.4%), and cefixime (81.7%). A significant association was observed between biofilm formation and resistance to most antibiotics, including nitrofurantoin, amoxicillin-clavulanate, amikacin, piperacillin-tazobactam, and sulfamethoxazole-trimethoprim, among others. Conclusion: These findings suggest that E. coli isolates resistant to these certain antibiotics are more likely to form biofilms. This research highlights the critical need to explore biofilm formation, along with antibiotic susceptibility patterns of isolates, to help select appropriate antibiotics and facilitate effective treatment approaches.

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