CLONING AND EXPRESSION OF UNIVERSAL STRESS PROTEIN 2 (USP2) GENE IN ESCHERICHIA COLI
Keywords:drought, heat, salt, Universal stress protein gene, gene manipulation, polygenic traits, stress tolerance
Different types of abiotic stresses inhibit the normal growth of plants by changing their physical biochemical, morphological, and molecular traits. It links to the polygenic traits, which is controlled with the help of different genes, due to this polygenetic the manipulation of foreign genetic makeup is very difficult. Drought stress is the very major type of threat to reduce the yield of cash crops in Pakistan and as well as in all over the world. Gene manipulation is the solution to face this problem by producing genetically modified crop plants that have the ability to survive in drought conditions. Universal stress protein gene has been already identified in bacteria which showed its response under stressed conditions, by manipulation of universal stress protein gene. It was found from our study that the bacterial cells transformed with the USP2 gene isolated from cotton induced abiotic stress tolerance under heat, osmotic, and salt stress. It was suggested from our findings that the USP2 gene could be used to produce abiotic stress tolerance transgenic crop plants to enhance crop plant yield and quality.
Adugna, W., Labuschagne, M., and Viljoen, C. (2006). The use of morphological and AFLP markers in diversity analysis of linseed. Biodiversity & Conservation 15, 3193-3205.
Deng, X., Long, S., He, D., Li, X., Wang, Y., Hao, D., Qiu, C., and Chen, X. (2011). Isolation and characterization of polymorphic microsatellite markers from flax (Linum usitatissimum L.). African Journal of biotechnology 10, 734-739.
Engel, E., Viargues, P., Mortier, M., Taillebourg, E., Couté, Y., Thevenon, D., and Fauvarque, M.-O. (2014). Identifying USPs regulating immune signals in Drosophila: USP2 deubiquitinates Imd and promotes its degradation by interacting with the proteasome. Cell Communication and Signaling 12, 41.
Fahramand, M., Mahmoody, M., Keykha, A., Noori, M., and Rigi, K. (2014). Influence of abiotic stress on proline, photosynthetic enzymes and growth. Int Res J Appl Basic Sci 8, 257-265.
Gelvin, S. B. (2003). Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool. Microbiol. Mol. Biol. Rev. 67, 16-37.
Grover, A. (2012). Plant chitinases: genetic diversity and physiological roles. Critical Reviews in Plant Sciences 31, 57-73.
Hanin, M., Volrath, S., Bogucki, A., Briker, M., Ward, E., and Paszkowski, J. (2001). Gene targeting in Arabidopsis. The Plant Journal 28, 671-677.
Hood, E. E., Helmer, G., Fraley, R., and Chilton, M. D. (1986). The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. Journal of Bacteriology 168, 1291-1301.
Howe, G. A., and Jander, G. (2008). Plant immunity to insect herbivores. Annu. Rev. Plant Biol. 59, 41-66.
Ibl, V., and Stoger, E. (2012). The formation, function and fate of protein storage compartments in seeds. Protoplasma 249, 379-392.
Isokpehi, R. D., Mahmud, O., Mbah, A. N., Simmons, S. S., Avelar, L., Rajnarayanan, R. V., Udensi, U. K., Ayensu, W. K., Cohly, H. H., and Brown, S. D. (2011). Developmental regulation of genes encoding universal stress proteins in Schistosoma mansoni. Gene regulation and systems biology 5, GRSB. S7491.
Jabeen, F., Shahbaz, M., and Ashraf, M. (2008). Discriminating some prospective cultivars of maize (Zea mays L.) for drought tolerance using gas exchange characteristics and proline contents as physiological markers. Pak. J. Bot 40, 2329-2343.
Jaleel, C. A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H. J., Somasundaram, R., and Panneerselvam, R. (2009). Drought stress in plants: a review on morphological characteristics and pigments composition. Int. J. Agric. Biol 11, 100-105.
Jin, H.-S., Hong, K.-W., Lim, J.-E., Hwang, S.-Y., Lee, S.-H., Shin, C., Park, H. K., and Oh, B. (2010). Genetic variations in the sodium balance-regulating genes ENaC, NEDD4L, NDFIP2 and USP2 influence blood pressure and hypertension. Kidney and Blood Pressure Research 33, 15-23.
Lawrence, S. D., and Novak, N. G. (2006). Expression of poplar chitinase in tomato leads to inhibition of development in Colorado potato beetle. Biotechnology letters 28, 593-599.
Li, H., and Greene, L. H. (2010). Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding. PloS one 5, e8654.
Li, J., Vaidya, M., White, C., Vainstein, A., Citovsky, V., and Tzfira, T. (2005). Involvement of KU80 in T-DNA integration in plant cells. Proceedings of the National Academy of Sciences 102, 19231-19236.
Lloyd, A., Plaisier, C. L., Carroll, D., and Drews, G. N. (2005). Targeted mutagenesis using zinc-finger nucleases in Arabidopsis. Proceedings of the National Academy of Sciences 102, 2232-2237.
Mahajan, S., and Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of biochemistry and biophysics 444, 139-158.
Majid, M. U., Awan, M. F., Fatima, K., Tahir, M. S., Ali, Q., Rashid, B., Rao, A. Q., Nasir, I. A., and Husnain, T. (2017). Genetic resources of chili pepper (Capsicum annuum L.) against Phytophthora capsici and their induction through various biotic and abiotic factors. Cytology and Genetics 51, 296-304.
Miki, B., and McHugh, S. (2004). Selectable marker genes in transgenic plants: applications, alternatives and biosafety. Journal of biotechnology 107, 193-232.
Pouly, D., Debonneville, A., Ruffieux-Daidié, D., Maillard, M., Abriel, H., Loffing, J., and Staub, O. (2013). Mice carrying ubiquitin-specific protease 2 (Usp2) gene inactivation maintain normal sodium balance and blood pressure. American journal of physiology-renal physiology 305, F21-F30.
Qamar, Z., Riaz, S., Nasir, I. A., Ali, Q., and Husnain, T. (2017). Transformation and evaluation of different transgenic lines for Glyphosate tolerance and cane borer resistance genes in sugarcane (Saccharum officinarum L.). Cytology and genetics 51, 401-412.
Reyes, F. C., Sun, B., Guo, H., Gruis, D. F., and Otegui, M. S. (2010). Agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology. Plant physiology 153, 624-631.
Ribas, A. F., Pereira, L. F. P., and Vieira, L. G. E. (2006). Genetic transformation of coffee. Brazilian Journal of Plant Physiology 18, 83-94.
Robinson, J. (2016). Audience Attitude Development and Maintenance in Political Talk Radio, Fielding Graduate University.
Rossier, B. C., Staub, O., and Hummler, E. (2013). Genetic dissection of sodium and potassium transport along the aldosterone‐sensitive distal nephron: Importance in the control of blood pressure and hypertension. FEBS letters 587, 1929-1941.
Shinozaki, K., and Yamaguchi-Shinozaki, K. (2007). Gene networks involved in drought stress response and tolerance. Journal of experimental botany 58, 221-227.
Sivamani, E., Bahieldin, A., Wraith, J. M., Al-Niemi, T., Dyer, W. E., Ho, T.-H. D., and Qu, R. (2000). Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant science 155, 1-9.
Sultan, M. T., Butt, M. S., Anjum, F. M., Jamil, A., Akhtar, S., and Nasir, M. (2009). Nutritional profile of indigenous cultivar of black cumin seeds and antioxidant potential of its fixed and essential oil. Pak J Bot 41, 1321-1330.
Tranel, P. J., and Wright, T. R. (2002). Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Science 50, 700-712.
Van Loon, A., and Van Lanen, H. (2013). Making the distinction between water scarcity and drought using an observation‐modeling framework. Water Resources Research 49, 1483-1502.
Van Loon, A. F., Gleeson, T., Clark, J., Van Dijk, A. I., Stahl, K., Hannaford, J., Di Baldassarre, G., Teuling, A. J., Tallaksen, L. M., and Uijlenhoet, R. (2016). Drought in the Anthropocene. Nature Geoscience 9, 89.
Wang, W., Vinocur, B., and Altman, A. (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218, 1-14.
Yang, Y., Duguay, D., Fahrenkrug, J., Cermakian, N., and Wing, S. S. (2014). USP2 regulates the intracellular localization of PER1 and circadian gene expression. Journal of biological rhythms 29, 243-256.
Zhu, T., Mettenburg, K., Peterson, D. J., Tagliani, L., and Baszczynski, C. L. (2000). Engineering herbicide-resistant maize using chimeric RNA/DNA oligonucleotides. Nature Biotechnology 18, 555.
How to Cite
Copyright (c) 2021 A Akram, K Arshad, MN Hafeez
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.