ANTIMICROBIAL SUSCEPTIBILITY PATTERNS OF PSEUDOMONAS AEUROGINOSA ISOLATED FROM PATIENTS WITH RESPIRATORY DISEASES IN A TERTIARY CARE HOSPITAL

H SAEED; Z RAMZAN; AW KHAN

doi:10.54112/bcsrj.v2023i1.317

Authors

H SAEED Department of Anesthesia, Allama Iqbal Memorial Teaching Hospital Sialkot, Pakistan
Z RAMZAN Department of Nephrology, Fatima Jinnah Medical University (FJMU), Lahore, Pakistan
AW KHAN Department of Pulmonology, Allama Iqbal Memorial Teaching Hospital Sialkot, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2023i1.317

Keywords:

Isolates, Sputum Culture, Antibiotic susceptibility, Pseudomonas aeruginosa

Abstract

Antimicrobial medicines have been extensively used since ancient times to treat various microbial infections, many of which can be life-threatening and contribute significantly to healthcare expenses worldwide. However, the increasing adaptability of these antibiotics has led to the emergence of resistance among microorganisms. This study aims to investigate the prevalence and susceptibility patterns of Pseudomonas aeruginosa among patients with respiratory disorders in a tertiary healthcare setting. A cross-sectional study was conducted at Civil Hospital Sialkot from September 2021 to June 2022, where 500 sputum samples were collected from both in and out-patients. Samples were collected following standard protocols and subjected to inoculation. Isolates were identified using standard biochemical protocols, and their antibiotic susceptibility patterns were determined using the Clinical and Laboratory Standards Institute (CLSI) guidelines and Kirby-Bauer's disc diffusion method. Out of the 500 sputum samples collected, 206 (41.2%) showed growth of microorganisms. Of these, 77 (37.38%) were identified as Pseudomonas aeruginosa. The isolates comprised 54.5% males and 45.5% females, with a mean age of 54.77 ± 11.16. The highest sensitivity was observed for Amikacin (93.5%), followed by Meropenem (92.21%), while the lowest sensitivity was observed for Cefoperazone (18.18%) and Tazocin (16.89%). Similarly, maximum resistance was seen in the case of Cefoperazone and Tazocin at (81.82%) and (83.11%) respectively. The results indicate that commonly used antibiotics against Pseudomonas aeruginosa are becoming increasingly resistant, with aminoglycosides and carbapenems remaining the two classes with the highest activity against this microorganism. These findings underscore the need for continued surveillance and appropriate use of antibiotics to minimize the development of resistance.

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References

Alam, M. S., Pillai, P. K., Kapur, P., and Pillai, K. K. (2011). Resistant patterns of bacteria isolated from bloodstream infections at a university hospital in Delhi. J Pharm Bioallied Sci 3, 525-30.

Church, D. L., Cerutti, L., Gurtler, A., Griener, T., Zelazny, A., and Emler, S. (2020). Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory. Clin Microbiol Rev 33.

Franco, B. E., Altagracia Martinez, M., Sanchez Rodriguez, M. A., and Wertheimer, A. I. (2009). The determinants of the antibiotic resistance process. Infect Drug Resist 2, 1-11.

Gill, M. M., Usman, J., Kaleem, F., Hassan, A., Khalid, A., Anjum, R., and Fahim, Q. (2011). Frequency and antibiogram of multi-drug resistant Pseudomonas aeruginosa. J Coll Physicians Surg Pak 21, 531-4.

Lambert, M., Smit, C. C. H., De Vos, S., Benko, R., Llor, C., Paget, W. J., Briant, K., Pont, L., Van Dijk, L., and Taxis, K. (2022). A systematic literature review and meta-analysis of community pharmacist-led interventions to optimise the use of antibiotics. Br J Clin Pharmacol 88, 2617-2641.

Pachori, P., Gothalwal, R., and Gandhi, P. (2019). Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes Dis 6, 109-119.

Poole, K. (2005). Aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 49, 479-87.

Poole, K. (2011). Pseudomonas aeruginosa: resistance to the max. Front Microbiol 2, 65.

Rajat, R. M. G., L Ninama; Kalpesh, Mistry; Rosy, Parmar; Kanu, Patel; Vegad, MM (2012). ANTIBIOTIC RESISTANCE PATTERN IN PSEUDOMONAS AERUGINOSA SPECIES ISOLATED AT A TERTIARY CARE HOSPITAL, AHMADABAD. National Journal of Medical Research 2, 156-159.

S, S., Reddy, A. S., S, S., C, A., V, S., Ms, K., Sk, A., and V, V. (2014). Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India. J Clin Diagn Res 8, DC30-2.

Samad, A., Ahmed, T., Rahim, A., Khalil, A., and Ali, I. (2017). Antimicrobial susceptibility patterns of clinical isolates of Pseudomonas aeruginosa isolated from patients of respiratory tract infections in a Tertiary Care Hospital, Peshawar. Pak J Med Sci 33, 670-674.

Steinberg, L., and Monahan, K. C. (2007). Age differences in resistance to peer influence. Dev Psychol 43, 1531-1543.

Zhao, W. H., and Hu, Z. Q. (2010). Beta-lactamases identified in clinical isolates of Pseudomonas aeruginosa. Crit Rev Microbiol 36, 245-58.