APPLICATION OF CRISPR TO INCREASE MEAT YIELD IN CHICKEN

U RAUF; F BATOOL; YA NOOR; M USMAN; KA SHAH; M IMRAN; M AHMAD; SMZ QOUSAIN; SUEF KAZMI; H ZAMAN

doi:10.54112/bcsrj.v2024i1.1079

Authors

U RAUF Veterinary Research Institute, Zarar Shaheed Road Lahore cant, Punjab Pakistan
F BATOOL Department of Poultry Sciences, MNS-University of Agriculture Multan, Pakistan
YA NOOR Department of Poultry Production, University of Veterinary and Animal Sciences, Ravi Campus, Pakistan
M USMAN Department of Poultry Science, University of Agriculture Faisalabad, Sub Campus Toba Tek Singh, Pakistan
KA SHAH Department of Veterinary and Animal Sciences, The University of Veterinary and Animal Sciences Swat, Pakistan
M IMRAN Livestock and Dairy Development (L&DD) Punjab, Pakistan
M AHMAD Poultry Research Institute Rawalpindi, Livestock and Dairy Development Department Punjab, Pakistan
SMZ QOUSAIN Department of Zoology, wildlife and fisheries, MNS-University of Agriculture Multan, Pakistan
SUEF KAZMI Department of Zoology, wildlife and fisheries, MNS-University of Agriculture Multan, Pakistan
H ZAMAN Department of Poultry Sciences, MNS-University of Agriculture Multan, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2024i1.1079

Keywords:

CRISPR Cas9, Poultry industry, Chicken production, Meat yield, PGS’s MSTN gene, Chicken

Abstract

Meat production is one of the major purposes of the poultry industry. Meat is full of protein and accomplishes the protein requirement of the diet. This study was carried out to attempt to use CRISPR-mediated genome editing of chicken to increase meat yield. For this purpose, CRISPR was used to edit the gene sequence and then it was allowed to infect the PGC’s (Primordial germ cells). The successfully edited PGC’s having blocked exon 3 of MSTN gene were then injected into the blood bloodstream of 2.6 days of fertilized egg cells. The eggs were incubated up to hatch. The wild type was taken as control and the Mutated ones as T1. The CRD was used with three replications to find the significance of variation in To and T1. The weight of chicks for T0 (wild type) and T1 (Mutated) was significantly different. The average weight of three replications of each treatment was compared through a bar graph. it was found that the CRISPR-mediated edited chicks were more weighted than that of their wild types. The CRISP-mediated inhibition of MSTN has a significant effect on muscle growth in chickens. The more such genes may be targeted to get more meat yield.

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References

Doudna, J. A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096.

Fiems, L. O. (2012). Double muscling in cattle: genes, husbandry, carcasses and meat. Animals, 2(3), 472-506.

Gerber, P. J., Steinfeld, H., Henderson, B., Mottet, A., Opio, C., Dijkman, J., ... & Tempio, G. (2013). Tackling climate change through livestock: a global assessment of emissions and mitigation opportunities (101-115).

Grobet, L., Royo Martin, L. J., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., ... & Georges, M. (1997). A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle. Nature genetics, 17(1), 71-74.

Hai, T., Teng, F., Guo, R., Li, W., & Zhou, Q. (2014). One-step generation of knockout pigs by zygote injection of CRISPR/Cas system. Cell research, 24(3), 372-375.

Kalds, P., Zhou, S., Huang, S., Gao, Y., Wang, X., & Chen, Y. (2023). When less is more: targeting the Myostatin gene in livestock for augmenting meat production. Journal of Agricultural and Food Chemistry, 71(10), 4216-4227.

Li, J., Han, Z., Zhao, Y., Liu, Y., & Xi, Q. (2018). CRISPR/Cas9-mediated knockout of myostatin in chicken DF1 cells induces myogenic differentiation via the TGF-β/Smad signaling pathway. Journal of Integrative Agriculture, 17(6), 1205-1212.

Mottet, A., & Tempio, G. (2017). Global poultry production: current state and future outlook and challenges. World's poultry science journal, 73(2), 245-256.

Qian, L., Tang, M., Yang, J., Wang, Q., Cai, C., Jiang, S., ... & Cui, W. (2015). Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. Scientific reports, 5(1), 14435.

Van Eenennaam, A. L., & Young, A. E. (2015). Animal agriculture and the importance of agnostic governance of biotechnology. Agriculture & Food Security, 4, 1-10.

Wang, K., Ouyang, H., Xie, Z., Yao, C., Guo, N., Li, M., ... & Pang, D. (2015). Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Scientific reports, 5(1), 16623

Yang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013). One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell, 154(6), 1370-1379.