OCCURRENCE OF TINEA INFECTION WITH COMPARATIVE STUDY OF COMMERCIAL ANTIFUNGAL AND TRADITIONAL HERBS IN DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN

MN UDDIN; B KHAN; M SHAH; S AHMAD; A ALI; T KHAN; . NIQABULLAH; S KHAN

doi:10.54112/bcsrj.v2024i1.967

Authors

MN UDDIN Centre for Biotechnology and Microbiology (CB&M), University of Swat, Pakistan
B KHAN Centre for Biotechnology and Microbiology (CB&M), University of Swat, Pakistan
M SHAH Centre for Biotechnology and Microbiology (CB&M), University of Swat, Pakistan
S AHMAD Centre for Biotechnology and Microbiology (CB&M), University of Swat, Pakistan
A ALI Riphah International University Islamabad, Pakistan
T KHAN Riphah International University Islamabad, Pakistan
. NIQABULLAH Department of General Medicine, Semey Medical University Kazakhstan, 071400, Abai Region, Semey City, 103 Abay St. Kazakhstan
S KHAN Centre for Biotechnology and Microbiology, University of Swat, Pakistan

DOI:

https://doi.org/10.54112/bcsrj.v2024i1.967

Keywords:

Tinea infection, dermatophytic infections, Anti-fungal, Traditional herbs

Abstract

Dermatophytosis, mainly Tinea infections, is caused by more than 30 species of dermatophytes. Tinea infections are usually restricted to superficial skin, but it has the potential for systemic infection, particularly in immune-compromised patients. The use of plant extract against tinea infection is well documented. However, limited studies have been conducted on the comparative studies of commercial antifungal drugs and traditional herbs. Objective: The current study was aimed to pinpoint the prevalence of tinea infection-causing pathogens, a comparative study of commercial antifungal drugs and traditional herbs against two fungal species, Trichophyton rubrum, and Aspergillus fumigatus isolated from dermatophytosis patients in Swat. Methodology: 190 samples were collected from infected peoples' skin, nails, and hairs in different hospitals and private medical facilities. The samples were cultured on potato dextrose medium, labeled carefully, and incubated. Moreover, the growths were observed under a microscope, and species were identified based on morphological characteristics. Results: A total of 12 different fungal species were isolated. Among all T. rubrum species, 25% was recorded, followed by Candida (19.4%) and Penicillium spp (16.6%). The minimum rate was recorded for Aureobasidium pullulans, Epidermphyton floccosum, Trichophyton basicola, T. verrucosum, T. tonsurans, and T. consultants with 2.78% each. A total of six anti-fungals were examined, and fluconazole and clotrimazole showed the best results against T. rubrum and A. fumigatus. Eight traditional herbs were studied against T. rubrum and A. fumigatus. Ethyl acetate extract showed the best results against both species, followed by methanol extract. n Hexane extract was found to be less effective. Conclusion: The study concluded that fluconazole, clotrimazole, and Ethyl acetate extract of medicinal plants were more effective against T. rubrum and A. fumigatus.

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References

White TC, Oliver BG, Gräser Y, Henn MR. Generating and testing molecular hypotheses in the dermatophytes. Eukaryotic cell. 2008;7(8):1238-45.

Rodwell GE, Bayles CL, Towersey L, Aly R. The prevalence of dermatophyte infection in patients infected with human immunodeficiency virus. International journal of dermatology. 2008;47(4):339-43.

Nigam PK. Antifungal drugs and resistance: Current concepts. Our Dermatology Online. 2015;6(2):212.

Banerjee M, Ghosh AK, Basak S, Das KD, Gangopadhyay DN. Comparative evaluation of effectivity and safety of topical amorolfine and clotrimazole in the treatment of tinea corporis. Indian journal of dermatology. 2011;56(6):657-62.

Godoy-Martinez P, Nunes FG, Tomimori-Yamashita J, Urrutia M, Zaror L, Silva V, et al. Onychomycosis in São Paulo, Brazil. Mycopathologia. 2009;168:111-6.

Graser Y, Kuhnisch J, Presber W. Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum. Journal of clinical microbiology. 1999;37(11):3713-7.

Uddin MN, Shah FA, Muhammad M, Aziz F, Din NU. Occurrence of tinea infection with comparative study of commercial antifungal and traditional herbs in district Swat, Khyber Pakhtunkhwa, Pakistan. 2024.

Saadabi AM. Antifungal activity of some saudi plants used in traditional medicine. 2006.

Shelef L, Naglik O, Bogen D. Sensitivity of some common food‐borne bacteria to the spices sage, rosemary, and allspice. Journal of Food Science. 1980;45(4):1042-4.

Shelef L. Antimicrobial effects of spices 1. Journal of food safety. 1984;6(1):29-44.

Aly M, Bafeel S. Screening for antimicrobial activity of some medicinal plants in Saudi Arabia. African Journal of Traditional, Complementary and Alternative Medicines. 2009:480-1.

Griffith GW, Easton GL, Detheridge A, Roderick K, Edwards A, Worgan HJ, et al. Copper deficiency in potato dextrose agar causes reduced pigmentation in cultures of various fungi. FEMS microbiology letters. 2007;276(2):165-71.

Khan W, Bakht J, Shafi M. Antimicrobial potentials of different solvent extracted samples from Physalis ixocarpa. Pakistan Journal of Pharmaceutical Sciences. 2016;29(2).

Havlickova B, Czaika VA, Friedrich M. Epidemiological trends in skin mycoses worldwide. Mycoses. 2008;51:2-15.

Irum F, Suhail M, Abro H. Keratinophilic fungi from the soil of district, Jamshoro, Sindh, Pakistan. Pakistan Journal of Botany. 2007;39(4):1377.

Adamski Z, Batura–Gabryel H. Medical mycology for physicians and students. Scientific Publishing of Poznan Medical University, Poznan, Poland; 2007.

Higgins E, Fuller L, Smith C. Guidelines for the management of tinea capitis. British Journal of Dermatology. 2000;143(1):53-8.

Paulo UdSPIdMTdS. Revista do Instituto de Medicina Tropical de São Paulo: O Instituto; 2005.

Zaugg C, Monod M, Weber J, Harshman K, Pradervand S, Thomas J, et al. Gene expression profiling in the human pathogenic dermatophyte Trichophyton rubrum during growth on proteins. Eukaryotic cell. 2009;8(2):241-50.

Youssef N, Wyborn C, Holt G, Noble W, Clayton YM. Antibiotic production by dermatophyte fungi. Microbiology. 1978;105(1):105-11.

Piérard GE, Arrese JE, Piérard-Franchimont C. Treatment and prophylaxis of tinea infections. Drugs. 1996;52:209-24.

Macura AB. Dermatophyte infections. International journal of dermatology. 1993;32(5).

Merlin K, Kilkenny M, Plunkett A, Marks R. The prevalence of common skin conditions in Australian school students: 4 Tinea pedis. British Journal of Dermatology. 1999;140(5):897-901.

Gupta AK, Cooper EA. Dermatophytosis (Tinea) and other superficial fungal infections. Diagnosis and treatment of human mycoses: Springer; 2008. p. 355-81.

Ameen M. Epidemiology of superficial fungal infections. Clinics in dermatology. 2010;28(2):197-201.

Cervelatti EP, Ferreira-Nozawa MS, Aquino-Ferreira R, Fachin AL, Martinez-Rossi NM. Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum. Genetics and Molecular Biology. 2004;27:99-102.

Fernández-Torres B, Carrillo A, Martın E, Del Palacio A, Moore M, Valverde A, et al. In vitro activities of 10 antifungal drugs against 508 dermatophyte strains. Antimicrobial agents and chemotherapy. 2001;45(9):2524-8.

Esteban A, Abarca M, Cabañes FJ. Comparison of disk diffusion method and broth microdilution method for antifungal susceptibility testing of dermatophytes. Medical mycology. 2005;43(1):61-6.

Ahmed AA, Bishr MM, El-Shanawany MA, Attia EZ, Ross SA, Paré PW. Rare trisubstituted sesquiterpenes daucanes from the wild Daucus carota. Phytochemistry. 2005;66(14):1680-4.

Aiyegoro O, Afolayan A, Okoh A. In vitro time kill assessment of crude methanol extract of Helichrysum pedunculatum leaves. African Journal of Biotechnology. 2008;7(11).