RETROSPECTIVE STUDY EVALUATING THE EFFICACY OF LJ MEDIUM ALONG WITH PENICILLIN TO IMPROVE CULTURE YIELD OF MYCOBACTERIUM TUBERCULOSIS IN PATIENTS

Authors

  • AA BUKHARI Microbiologist & Senior Demonstrator, Pathology department, MMDC Multan
  • MSN KHAN Assistant Professor, Medicine, MMDC Multan
  • A AKRAM Microbiology, MMG, Visiting Lecturer at Microbiology Department, Emerson University Multan
  • AA BUKHARI Public Health Consultant, Family Medicine Specialist, X-Centre Head Concept Fertility Centre Multan
  • N NAEEM Pathology department, Faisalabad Medical University
  • A NAZ Pathology department, MMDC Multan

DOI:

https://doi.org/10.54112/bcsrj.v2023i1.327

Keywords:

Mycobacterium Tuberculosis, Pulmonary Tuberculosis, Penicillin, Lowenstein Jensen Media

Abstract

This study evaluated the efficacy of penicillin in improving the culture yield of M. Tuberculosis in cases of pulmonary tuberculosis. A retrospective study was conducted in the Department of Pathology, MMDC, Multan. A total of 350 suspected pulmonary tuberculosis patients were selected for the study. An early morning sputum specimen was collected. NAOH citrate and N-acetyl-L-cysteine were used for digesting, decontaminating, and homogenizing the sputum. The samples were processed on Lowenstein Jensen media and then Lowenstein Jensen media with penicillin. The cultures were then examined after 2 months. On LJ media, 100 (28.5%) suspected cases were positive, and 80 (22.8%) cases were contaminated. On LJ media with penicillin, 145 (41.4%) cases were positive, and 15 (4.3%) were contaminated. In addition, 30 (8.5%) were 1+ grading, 15 (4.3%) were 2+ grading on both media, 95 (27.1%) were 3+ grading on LJ media, and 50 (14.2%) were 3+ grading on LJ media with penicillin. Contamination in Mycobacterium tuberculosis is reduced by adding penicillin to the LJ media, and isolation-positive cultures are enhanced.

Downloads

Download data is not yet available.

References

Chihota, V., Grant, A., Fielding, K., Ndibongo, B., Van Zyl, A., Muirhead, D., and Churchyard, G. (2010). Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting. The international journal of tuberculosis and lung disease 14, 1024-1031.

Glaziou, P., Floyd, K., and Raviglione, M. C. (2018). Global epidemiology of tuberculosis. In "Seminars in respiratory and critical care medicine", Vol. 39, pp. 271-285. Thieme Medical Publishers.

Hertz, D., and Schneider, B. (2019). Sex differences in tuberculosis. In "Seminars in immunopathology", Vol. 41, pp. 225-237. Springer.

Kassaza, K., Orikiriza, P., Llosa, A., Bazira, J., Nyehangane, D., Page, A.-L., and Boum, Y. (2014). Lowenstein-Jensen selective medium for reducing contamination in Mycobacterium tuberculosis culture. Journal of Clinical Microbiology 52, 2671-2673.

Ma, Y., Fan, J., Li, S., Dong, L., Li, Y., Wang, F., Huo, F., Pang, Y., and Qin, S. (2020). Comparison of Lowenstein-Jensen medium and MGIT culture system for recovery of Mycobacterium tuberculosis from abscess samples. Diagnostic Microbiology and Infectious Disease 96, 114969.

Rasool, G., Khan, A. M., Mohy-Ud-Din, R., and Riaz, M. (2019). Detection of Mycobacterium tuberculosis in AFB smear-negative sputum specimens through MTB culture and GeneXpert® MTB/RIF assay. International journal of immunopathology and pharmacology 33, 2058738419827174.

Selmane, S., and L'Hadj, M. (2020). Epidemiology and clinical characteristics of tuberculosis in leon bernard tuberculosis unit in algeria. The International Journal of Mycobacteriology 9, 254-260.

Suárez, I., Fünger, S. M., Kröger, S., Rademacher, J., Fätkenheuer, G., and Rybniker, J. (2019). The diagnosis and treatment of tuberculosis. Deutsches Aerzteblatt International 116.

Tahseen, S., Khanzada, F. M., Baloch, A. Q., Abbas, Q., Bhutto, M. M., Alizai, A. W., Zaman, S., Qasim, Z., Durrani, M. N., and Farough, M. K. (2020). Extrapulmonary tuberculosis in Pakistan-A nation-wide multicenter retrospective study. PloS one 15, e0232134.

THANGAVELU, K., JAMIR, I., ELLAPPAN, K., KRISHNAKUMARIAMMA, K., GOPICHAND, P., DAS, S., and JOSEPH, N. (2021). Comparison of MGIT 960 with Lowenstein Jensen Media for Recovery of Mycobacteria from Extrapulmonary Specimens in Southern India. Journal of Clinical & Diagnostic Research 15.

Tharmalingam, D., Kopula, S. S., and Palraj, K. K. (2019). Evaluation of thin-layered agar for Mycobacterium tuberculosis isolation and drug susceptibility testing. The International Journal of Mycobacteriology 8, 153-156.

Umar, F., Hatta, M., Husain, D. R., Natzir, R., Dwiyanti, R., Junita, A. R., and Primaguna, M. (2019). The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients. New Microbes and New Infections 32, 100609.

Yen, Y.-F., Hu, H.-Y., Lee, Y.-L., Ku, P.-W., Ko, M.-C., Chuang, P.-H., Lai, Y.-j., and Chu, D. (2018). Sexual inequality in incident tuberculosis: a cohort study in Taiwan. BMJ open 8, e020142.

Downloads

Published

2023-06-27

How to Cite

BUKHARI, A., KHAN, M., AKRAM, A., BUKHARI , A., NAEEM, N., & NAZ, A. (2023). RETROSPECTIVE STUDY EVALUATING THE EFFICACY OF LJ MEDIUM ALONG WITH PENICILLIN TO IMPROVE CULTURE YIELD OF MYCOBACTERIUM TUBERCULOSIS IN PATIENTS. Biological and Clinical Sciences Research Journal, 2023(1), 327. https://doi.org/10.54112/bcsrj.v2023i1.327