Biological and Clinical Sciences Research Journal
ISSN: 2708-2261
www.bcsrj.com
DOI: https://doi.org/10.47264/bcsrj0101035
Biol. Clin.
Sci. Res. J., Volume, 2020: e035
Review Article
ROLE OF RHIZOBACTERIA IN PHYTOREMEDIATION OF HEAVY
METALS
NADEEM N1, ASIF R1, AYYUB S1,
SALMAN S2, SHAFIQUE F1,*ALI Q1, MALIK A1
1Institute
of Molecular Biology and Biotechnology, The University
of Lahore, Lahore, Pakistan
2Department
of Plant Breeding and Genetics, Faculty of Agriculture, Gomal
University, DI Khan, Pakistan
*The
first four authors; Nadeem N, Asif
R, Ayyub S and Salman S have
equal contribution towards manuscript
*Corresponding author email: saim1692@gmail.com
Abstract: Rhizobacteria, a plant growth promoting rhizobacteria (PGPR) as beneficial microorganism which
helps in defense from abiotic and abiotic
stresses, colonizes in rhizosphere and played a major
role in promoting plant growth and also provides enhance soil fertility. In
the highly contaminated soil, the content of metal exceeds the limits of plant tolerance.
It is also possible that treatment of plant with PGPR, here increasing the
biomass of plant, stabilizing and the remediation of metal polluted soil. The
use of rhizobacteria plays and important role in
increasing the tolerance of plant towards toxic effects of heavy metals like
arsenic, sulphur, mercury, chromium, cadmium, nickel, lead and copper etc. Heavy
metal accumulation results in deterioration of soil fertility while PGPR helps
to restore soil fertility. The process of phytoremediation
has been proved to be the best way to remediate heavy metals from soil. The use
of rhizobacteria with plants provides highly
efficiency phytoremediation. However, there is still
need to understanding the concept of microbial ecological study in rhizosphere and mechanism of detoxification of heavy metals
form rhizosphere.
Keywords: rhizobacteria, heavy metals, plant growth
promoting bacteria, phytoremediation, abiotic stress
Introduction
Heavy
metals may be defined as elements which have metallic features like
conductivity, ligand specificity, stability, etc.
Metals have been present naturally in the soil with a lot of heavy metals ions
accumulated in the form of compounds, or as simple ions and which are essential
for plants as micronutrients (Rahmaty and
Khara, 2011; Sharma et al., 2003; Tahir et al., 2020). Due to
industrial revolution the pollution increased intensely by the toxic heavy
metals due to human activities such as electroplating, fuel production,
manufacturing and mining of metals, pesticide application etc (Almaroai et al., 2012; Arroyo et al., 2002; Babarinde et al.,
2006). Metal
pollution has become the most severe problem among the environmental problems
nowadays (Cao et al., 2004; Khalil et al., 2020; Seregin et al.,
2004; Zubair et al., 2016). Rhizobacteria is plant growth promoting (PGPR) beneficial
microorganism which helps in defense from abiotic and
abiotic stresses, colonizes in rhizosphere
and played a major role in promoting plant growth and also provides enhance
soil fertility (Lugtenberg
and Kamilova, 2009; Vessey, 2003).
Rhizosphere bacterial
species play an important role in phytoremediation
from heavy metals in contaminated soil, through releasing of chelating agents
heavy metals mobility affect the microbial population and also availability to
plant, release of redox changes, phosphate solubilization, acidification and promote phytoremediation (Iqra et al., 2020; Lugtenberg and Kamilova, 2009;
Mazhar et al., 2020b; Vessey, 2003). In phytoremediation process rhizobacteria
metal adapted able to received extra attention. We need to increase our
understanding about mechanisms involved in mobilization and transfer of heavy
metals in the contaminated soils (Antoun, 2013). Soils which are polluted with
heavy metals cause environmental problems the reason behind is the effects of
metals which is highly toxic. There have options for sediments polluted due to
metals and reclamation of soil is ex-situ and in-situ techniques (Bĺĺth, 1989;
Pamukcu and Kenneth Wittle, 1992;
Ross, 1994). The in-situ aims for
remediation from soil is that enhance the maintenance of metals either in soil
particles. The other methods work on plants to reduce bioavailability or
potential mobility of toxic metals from environment. The purpose of ex-situ
techniques is that separating and extracting of metals from the soil over
series of biological, physical and chemical methods with the help of
specifically designed reactor (Dixit et al., 2015; Jansen et al., 1994; Nouri et al.,
2009).
Phytoremediation method offers
detoxification and removal of contaminants which involved the use of plants to
sequester and it is very effective, ecologically gentle, less
expensive and also a socially standard technology specifically for pollution elimination
(Memon et al., 2001; Schröder et al., 2008). Phytoremediation depends upon the tolerance and ability of
plant to accumulate HMs (high concentrations) and also to obtain yield from a
large plant biomass (LeDuc and
Terry, 2005; Mench et al., 2009). Heavy metals mostly biologically
non-degradable but persist indefinitely mostly in under developed and developed
countries as contaminations in the environment. Presence of heavy metals in
plants harshly effects on growth and crop yield, symbiosis due to high
concentrations of heavy metals (Felix, 1997;
Giordano et al., 1975; Grytsyuk et al.,
2006). The heavy
metals are harmful for soil because the heavy metals cannot be degraded
biologically but it is likely to transfer from one oxidation state to the
alternative state which is less toxic form of the compound (Chu and Wong,
1987; Schmidt, 2003). Degrading capacity of rhizobacteria has been increased the spectrum by using or
introducing new techniques such as genetic engineering. phytohormones major chemical which are involved in
the uptake of metal. Rhizobacteria plays very
important role in phytoremediation and also release
essential hormones for plant growth (Garrido et al., 2005; Prasad et al., 2011).
The
heavy metal stress caused important impact on plants that’s why the plants have
to avoid it through some of the mechanisms such as.
1.
At
root surface metal ion adsorbed.
2.
Root
cells transversely membrane metals ions help to move into the root cells.
3.
In
vacuole metal ion immobilized in small proportion.
4.
Movement
of the metal intracellularly through the vascular
tissue of root
5.
The
accumulation of metallic ions through the root-to-shoot transfer and also from
leave tissue.
Mobilization
and immobilization of the heavy metals is one of the important roles of rhizobacteria which provide tolerance to heavy metals. Soil
pollution is the significant and serious environmental problem and cause
negative effect on agriculture as well as also on human health (Gadd, 2000;
Lahori et al., 2017; Unz and
Shuttleworth, 1996). The
significant interface of plant and soil is rhizobacteria,
which plays vital role in the phytoremediation of
polluted soils through remediating the heavy metals. Extreme gathering of the heavy
metals in several plants species has been found highly toxic (Grytsyuk et al., 2006; Krantev et al., 2008; Lahori et al.,
2017). Presence of
heavy metal ions at high level in the atmosphere absorbed from root and
transferred to the shoot which effect on plant growth and reduced metabolism.
Contamination of soil and water due to heavy metals is also a major problem.
Moreover, high concentrations of metal in the soil have become the reason of
many problems such as decrease in soil fertility, microbial action and also effect
on yield production (Henning et al., 2001; Iqra et al., 2020; Ma et al., 2003). Cadmium is a
toxic and non-essential heavy metal which is able to reveal the problem and also
inhibits shoot and root growth, nutrient uptake disturbs and accumulated rate of
important crops. The crop plants which are usually rich in Cadmium and consumed
by human and animals causes many harmful diseases. On the other hand, if Cd concentration not controlled then the overtime soil may
be ultimately become unstable for the production of crop plants (Das et al., 1997; Hasan et al., 2009; Hou et al., 2007). Heavy metals
cause contamination in environment treated by conventional technologies which
is based on the physicochemical principles but these technologies are
uneconomic and inefficient. On the other hand, removal of metals from aqueous
solution is carried with the addition of reagents in the solution which
increase the pH and soluble form of metals converts into the insoluble form
which causes precipitation in the solution (Cuypers et al., 2011; Martelli et al., 2006; Nagajyoti et al.,
2010).
Interactions
of rhizobacteria
Interaction
between plant and bacteria
Root
zone of plants colonize with dense population of microorganisms. Rhizosphere is very attractive habitat as compare to bulk
soil, organic carbon which is gained from the plant roots (Etesami, 2018;
Van Loon, 2007). In Rhizosphere
more than 85% organic carbon can be originated from the tissues and sloughed
off the root cells. Relationship between plant and bacteria is always naturally
symbiotic in this interaction both of them get benefit from each other. In this
relationship roots of plants are mainly involved to give benefit to the
bacteria (Antoun and
Prévost, 2005; Chen et al., 2000; Persello‐Cartieaux et al., 2003). Similarly,
bacteria help plant in the maintenance of nutrient supply in and in recycling
process. Rhizobacteria also maintain soil fertility
and also detoxified harmful chemicals which usually released due to toxicity of
heavy metals. Plants provide carbon source to the bacteria which reduce phytotoxicity (Requena et al., 1997; Zahran, 1999).
Plant bacteria interaction
with respect to soil
Composition
of root and soil conditions plays an important role in the interactions with respect
to specificity. Rhizobacteria called as soil
tolerated bacteria because it inhibiting rhizosphere.
Some of the bacterial species also colonized around the surroundings and to the
surface of root such as the endobacteria (Barea et al., 2005; Nadeem et al., 2014; Requena et al.,
2001). The
interaction can be specific as well as non-specific. Toxic metal inhibits the
growth of plant but other factors like water, low-soil fertility, beneficial
nutrients, harsh and dry conditions may also be responsible for the inhibition
of plant growth (Bhattacharyya
and Jha, 2012; Haas and
Défago, 2005).
Removal of heavy
metals and PGPR interaction
The
removal of heavy metals is very important due to their toxicity. Release of
phosphate solubilization, acidification process,
chelating agents and redox changes enhance the
potential of phytoremediation. There is a need to progress
understanding about mechanisms which are involved in the mobilization and also
in the transfer of heavy metal so, we can be able to reduce the heavy metals
from soil (de Oliveira Mendes et al., 2014; Goldstein, 1995; Halder and Chakrabartty, 1993; Khan et al., 2009). Heavy metals
disturb the metabolic processes of plant and animals. Interaction of bacteria
with plant affected from these following conditions which are very important
for plant and bacteria.
1.
Improper
supply of water.
2.
Harsh
climate changes.
3.
Deficiency
of soil fertility.
4.
Lack
of nutrients.
Heavy metals
effect on plants
For
plant uptake heavy metals present in the soil as soluble components. Some heavy
metals require for growth of plant and also for maintenance but if those metals
present in high range than it become toxic for plant (Dubey, 2010;
McIntyre, 2003; Reichman, 2002). And cause cytoplasmic
enzymes inhibition due to oxidative stress damage cell structures. If heavy metals
cannot remove from plant than ultimately plant died due to its toxic effects.
Copper is important metal for development and growth of plant. The plant growth
promoting rhizobacteria (PGPR) are beneficial for the
plants and helps to reduce the toxicity level. The higher concentration and
long term presence of heavy metals plant become chloric
and cause deficiency of iron (Khalid et al., 2015; Sayyed et al., 2013; Tank and Saraf, 2009).
Mechanism
Rhizobacteria secretions
Rhizobacteria secretion could
play a key role for phytoremediation which is assisted
by rhizobacteria. Direct mechanism contains nitrogen
fixation synthesis of siderophores or indirect
mechanism contains inhibiting phytopathogens from
plant growth then development (Kloepper et al., 1980; Van Loon et al., 1998). The microbes promote the plant
growth under stress conditions and help in degradation of contaminants. The PGPR
has been mostly used for an extensive period assisting plant to uptake large
amount of nutrients from soil or preventing plant diseases (Glick, 2012;
Saharan and Nehra, 2011; Schippers et al., 1987). PGPR
application has been prolonged to bioremediation of organic metal pollutants. Rhizobacteria create metal chelating agents called siderophores and some heavy metals they have main part in
acquisition. Organic matters have the result of scavenging Fe3+
increase the bioavailability of soil bound iron (Falkowski and
Raven, 2013; Hughes and
Poole, 1989; Schroth and
Hancock, 1982). Lived in the metal polluted
soils are often iron deficient in plant growth, the microbial siderophore are used in iron chelating agents they set the
accessibility of iron in the plant rhizosphere (Bruins et al., 2000; Gavrilescu, 2004; Raffi et al., 2010). The plants
required minor iron concentrations for the normal growth than do microbes but
binding affinity of Phyto siderophores
for iron is less than affinity of microbial siderophores.
Root growth stimulated by different species of plants and has 1-aminocyclopropane-1-carboxylate
(ACC) deaminase enzymes which control the amount of
ACC by decreasing or hydrolyzing and ethylene a plant hormone precursor
biosynthesis of plant by ethylene (Hontzeas et al., 2006; Ma et al., 2003; Madhaiyan et al.,
2007). Removal of ACC
from seeds or roots is taken up through the bacteria and cleaved by ACC deaminase to ammonia. The inner and outer level ACC the
plant need exude increase amount of ACC (Jia et al., 2000; Nascimento et al., 2014).
Transform toxic
heavy metals
The
decrease in growth due to heavy metals among 25% to 40% depending on the type
of metal both in root tissue as well as shoot when plants have ability to create
a very significant diminution of accumulation for heavy metals particularly in the
plant roots while than 50% decrease in addition of Cd,
Pb and Zn in the roots (Hansda et al., 2014; Mushtaq et al., 2020; Saleem et al.,
2007; Singh et al., 2019). The
bioavailability of metals in soil to plant is also influenced the phytoremediation to productivity of plant. Bacteria can
convert toxic heavy metals to form that are additional readily taken up into plant
roots. Bacteria can increase the concentration of Selenium and organ selenium
forms such as Smet are known to be taken at faster
rates (Khan and
Bano, 2018; Ojuederie and
Babalola, 2017). The comparative change of
organic bound with Cu, Pb and Zn were separately +5%
+3% and +23% while in the infected rhizosphere 0.8%,
-2% and -3% in the non-infected rhizosphere
respectively. So, a huge amount of Cu, Zn and Pb
bounded by organic matter in the infected rhizosphere
(Abbaszadeh-Dahaji et
al., 2016; Guarino et al., 2020). Chemical properties like pH of organic
matter are directly affected the metal bioavailability through moving their
soil rhizobacteria. The Pseudomonas melophilia
reduced the mobile and toxic Cr3+ into nontoxic Cr6+ and
also to reduce environmental mobility for additional toxic ions Hg2+,
Pb2+ (Jeevanantham et al., 2019; Li et al., 2019).
Inhibition of
plant pathogens
PGPR
provides different ways to suppress plant viruses. It involves the competition
of nutrients and an anti-bacterial environment for the manufacture of
antibiotics and the production of siderophores that
reduce the availability of the iron needed for bacterial growth (Glick and
Stearns, 2011; Ma et al., 2011; Tak et al., 2013). Another
mechanism similar to the production of lytic enzymes
B-1,3-glucanases and chitinases
plays a vital role in the reduction of glucan and
chitin in the fungal cell wall. PGPR showed resistance to heavy metal during phytoremediation process. It has been shown that
inoculating plants with plant growth that promotes rhizobacteria
or by treating microbes with plant microscopic plantings may be effective in promoting
plant growth (He and Yang,
2007; Kong and
Glick, 2017; Nie et al., 2002).
Stimulation of
transport protein
Transport
protein participating in the passage of ions. The protein might support in the
change of substances by means of facilitated diffusion. Plants flourishing in
tiny pots are usually considerably smaller than those expanding in large, at
the same time when they have superficially suitable sources of water and
nutrients (Salt et al., 1998; Singh et al., 2003; Zhao and McGrath, 2009). Bacteria
persistence and propagation in the atmosphere furthermore within numerous hosts
are censoriously reliant on the uptake and appropriation of conversion metals
like zinc, iron, and manganese. For instance, cells might rigorously
standardize intracellular zinc concentration while the elevated concentration
of zinc is always noxious for cellular purpose which developed numerous types
of protein implicated in attachment and transfer of zinc ions (Hall, 2002;
Pilon-Smits, 2005; Wuana and Okieimen, 2011). The bacteria
might also accelerate sulfate move proteins placed in the root cell or plasma
membrane which similarly stimulates selenite mineral.
The inorganic Hg absorbance elevated in plants has not been correctly inspected
but has been associated to the inactive acceptance of lipophilic
chloride facilities in phytoplankton (Hutchinson,
1973; Thomas et al., 1980).
Speciation
versus bioavailable of heavy metal in soils
Soil
rhizobacteria can also directly affect the melting of
iron by altering the specificity of heavy metals in rhizosphere.
The mycorrhiza played an important role in iron
deficiency from the rhizosphere and it has impact on
increasing plant tolerance in trapping heavy metals from soil (Li et al., 2015; Marschener, 1998; Watteau and Berthelin, 1990). Although high heavy
metal concentration is usually harmful to microbial physiological aspects, therefore
the tracking of heavy metals amount and number of different heavy metals is very
necessary for the normal bacterial growth for reducing redox
and cellular activity. Bacterial interactions with heavy metals depend on metal
forms and the availability of bioavailability (González-Guerrero et
al., 2016; Xie and Tang,
2019). The
interaction of plant bacteria can promote the production of chemicals that can
alter the chemical properties of the soil in rhizosphere
and improved the accumulation of heavy metals in the plants body. The
availability of bioavailability depends on a variety of factors such as soil
pH, cation exchange rate, soil content of organic
matter, mineral and iron content, water and heat content, biological properties
of soil, chemical properties of metals and microbial activities under soil and
climate conditions. In addition, the availability for heavy metal ions
increases under low anaerobic oxidizing pH condition (Joshi and
Juwarkar, 2009; Marsh Jr et al., 1963; Ullah et al., 2015).
PGPB mechanisms
to control heavy metals stress
The
heavy metals cannot be decomposed which can be harmless to bacteria. A few
microorganisms have evolved to develop ways of removing toxins from the towards
combat the harmless effects of these inanimate metals (Sgroy et al., 2009). Heavy metals such as Al, Pb, Cd, do not production any
role in nature and are harmless to living organisms. There are several ways to
protect against heavy metal resistance by microbial cells. These processes are
the outer barrier of cells, the outer division of cells, and the active
transport of metallic ions, the inner cell structure, and the reduction of metallic
ions (Ahemad, 2019;
Begum et al., 2019). Metal filtration that exceeds
biological requirements prevents the growth of bacteria or bacteria that react
to high levels of metals through various forms of resistance against heavy
metal toxicity. Bacterium which increase the growth of plants such as Rhizobium, Brad rhizobium and
Pseudomonas have been exposed to Co2+, Cu2+, Zn2+,
Mn2+, Fe2+, Mo2+ and the sensitivity of these
metals has been tested in vivo which has shown that Rhizobium
legumin Sarum spots are
very sensitive to Cu2+ and Co2+ as compare with rhizobium (Islam et al., 2016; Rajkumar and Freitas, 2008). This flexible metal resistance method
was tested by examining the areas exposed to anthropogenic or natural metal
contamination for a long time. Acquisition of heavy metals by microorganisms
occurs by bioaccumulation which is an active process or by adsorption which is
a synthetic process (Bashan and
De-Bashan, 2010; Dodd et al., 2010; El-Meihy et al., 2019). Many micro-organisms
such as fungi, bacteria and algae have been used to clean contaminating areas by
heavy metals. Bacteria use two types of heavy ion detection methods. The
initial mechanism of chemiosmosis gradient throughout
the cytoplasmic membrane is rapid and undetectable.
Another method is a specific substrate, which slows down and undergoes ATP
hydrolysis. The most important methods are physical isolation, exclusion and
difficulty (Bunow, 1978;
Lane et al., 2010; Silver and Phung, 2005; Taj and Rajkumar, 2016).
When
heavy metals attached to the extracellular cell material, they can immobilize
metals and blockage of its intake with the help of bacterium cells. On the cell
surface, most of metals bind to the functional group that is anionic. By
forming the effective barrier around the cell, heavy metals bind to
extracellular polymers i-e proteins, polysaccharide
etc. which helps to detoxify them. Siderophores
minimize the bioavailability of metal here performs the toxicity reducing.
Specific production metabolites result in precipitation of heavy metals (Ahemad, 2015;
Pajuelo et al., 2011; Sher and Rehman, 2019; Zubair et al.,
2016). Different
types of bacteria manifest efflux for the transporters with higher level of substrate
affinities because of which they can expel toxic metals with high concentrations
which expelled outside of the cell. The plasmid encoded energy dependent system
which involves chemiosmosis ion pumps. Along with the
ATPase that are reported for acceptance of cadmium
and chromium (De la Torre et al., 1999; Lakra et al., 2006; Pratap and Bonga, 1993). There is
another method for toxicity of heavy metals in which ions may be converted into
innocuous form which follow the entry into bacterial cells. This whole
mechanism is known as mechanism of cytosolic
sequestration. This allows the uptake of heavy metals with high concentration;
for instance, metallothionein’s synthesis. They have
low molecular weight. The metal binding protein that is cysteine
has high metal affinity e.g., copper, cadmium, silver and the mercury (Canli et al., 2001; Tanwir et al., 2015; Tiwari et al.,
2009). As an
alternative, some of the bacteria utilize methylation
method that involves the methyl group which is transferred to the metal and
metalloids. There is a limitation that only a few metals can be methylated. There is also a method used for the toxicity of
heavy metals is decontamination of soil which involved the heavy metals
reduction (Bordajandi et al., 2004; Karadede-Akin and Ünlü, 2007). The species of bacteria
responsible for the reduction of heavy metals referred as dissimulator of metal
reducing bacterium. Those bacterial strains help in detoxification of chromium which
involves the reduction of Cr by the strains of bacteria which plays a
significant role in the acquisition of severely heavy metals (Liu et al., 2006; Viti et al., 2003; Wang and Shen, 1995).
Significance of rhizobacteria in phytoremediation
For improving
the growth of plant e.g., wheat, we have to screen PGPR. The study has suggested
that the potential for biosynthesis of auxin through rhizobacteria which could be used as a tool for screening
the effective PGPR strains (Deshwal and Kumar, 2013; Wani et al.,
2009). A new combination of in vitro screening method which
include microplate assay with plant i-e strawberry seedling to test the PGP strain which have
more efficient potential biological controlling agents which has been developed
successfully. To screen the effective PGPR strain ACC deaminase
trait could be used as an efficient tool. It could be successfully used as
bio-fertilizers which increase the growth of inoculated plants (Mazhar et al.,
2020a; Wani et al., 2015). In the soil, the high levels of heavy metals
decrease the microbial activity and it affects the production of crop by
getting accumulated in plant organs. Metal ions are present in the soil and
these are absorbed by the roots which transported to various plant organs. The
enzymes and proteins in the cells which have higher affinity of heavy metals
they rendered them inactive and also lost their function (Hansda et al.,
2014; Liu et al., 2015). When they interact with heavy metals the structure
of protein change and affect the plant growth by photo-system inactivation. At
the end the free radicals produced oxidative effect that has adverse effect on
biochemical and the physiological processes of plants. Reduction in growth and
also development happens due to the disturbance in photosynthetic and also
respiratory mechanism (Chaudhary and Khan, 2018; Qi et al., 2018; Tirry et al.,
2018). The mechanism of PGPB to overcome the metallic
stress, bacteria including the PGPB have several resistance mechanisms they can
be immobilize, transform the metals, as well as reducing the toxicity which
tolerate the uptake of heavy metal ions. PGP Bacteria help the plants to grow
and these bacteria grow in soil or on the roots of plant. Pant will grow by
helping the certain nutrients, modulating the hormone levels of plant and also
protecting the plant from any type of pathogen (Saharan and Nehra, 2011; Zhu et al., 2015).
The quality of
bacteria that respond to heavy metals plays an important role in utilization
for bio-remediation of accumulation of toxic metals in plants. In agronomic
processes, these bacteria express the unique ability of metal detoxification
along with the growth promoting agent’s property (Ayangbenro and Babalola, 2017; Zaidi et al., 2009). Researchers had reported many applications of
bacteria i.e., sphingomonas macrogoldabidus,
micro bacterium lique-faciens and the micro bacterium
arabino-galactanolyticum inoculated to the plants;
the A-murale which gives significant results by
increased of Ni-uptake by plants as compared to the untreated plants. Carrillo-Castaneda
reported that the potential of PGP in the protection of alfalfa (Medicago sativa) seeds
from the accumulated copper due to the absorption by the roots to the shoots in
the seedlings of these plants (Silver and Phung, 2005; Vacheron et al.,
2013). It has been reported that Hydroxamate
siderophores shown that besides the presence of heavy
metals which increase the iron uptake by plants. Iron is the essential
micro-nutrients for plants and also for microbes. Under anaerobic conditions
such as flooded soil, high concentration of Fe2+ ions which are
generated through reduction of Fe3+ ions and due to excessive uptake
of iron leads to iron toxicity for plant cells. In aerobic conditions solubility
of iron is low here limiting the supply of iron for different forms of life (Van Loon, 2007; Watteau and Berthelin, 1990). Bacteria can overcome the limitation of nutritional
iron by using the chelatoragentsis known as siderophores. Through various ways the PGPB play a
beneficial role in the growth of plant. For example, from PGPB siderophores prevent some pathogens from sufficient amount
of iron hereby limiting the ability to proliferate. Nitrogen fixation is
another important role of PGPB in the field of biology. Under the conditions of low soil moisture,
the rhizobia are sensitive to drought stress resulting the decrease in nitrogen fixation. These studies
gave an insight to the role PGPB, under the heavy metals stress increasing the
biomass of plants (Saleem et al.,
2007; Salt et al., 1998; Sayyed et al., 2013).
Transformation
and the uptake of heavy metals
The mechanisms which
are involved in transformation of metal ions in soil leads to wards the loss of
heavy metals (uptake of plant, leaching and the volatilization reactions). The most
of the metals do not undergo the volatilization related losses. Actually, the
fate of metalloids in soil totally depends upon its properties and its
environmental factors (Khan and Bano, 2018; Marschener, 1998; Ross, 1994). The availability and the mobility of metals in soil
affected by microbes and they can be done through certain steps, acidification,
changes of redox, production of iron chelators and the siderophores,
mobilizing the metal phosphates. Actually the heavy metal in soil which are
bound to both organic and inorganic substances or these are present as insoluble
precipitates that are not available for root uptake, siderophores
of bacteria produce (Sayyed et al.,
2013) PGPB which have the ability to solubilize
the heavy metals having Fe and make them available for roots of plants to take
up. These PGPR helps to reduce the metals toxicity by bio-sorption method
because of the bacterial cells absorb high amount of heavy metals. The
environmental factors that affect the growth of plant include water, light, and
the temperature, nutrition and also humidity. Soil hardness affects the growth
of roots (Gadd, 2000; Haas and Défago, 2005; Khan and Bano, 2018).
Approach by genetically engineered
Various
genetically engineered approaches have been developed and these are used to
optimize the enzymes, organisms that are relevant for bio-degradation, and also
metabolic pathways. With the molecular method allow the characterization of
structure of microbial community and the activities. There are a large number
of proteins which bind to different heavy metals with whole range with greater
affinity (Saharan and Nehra, 2011; Vessey, 2003; Zaidi et al.,
2009). The metal binding proteins are at the outer
membranes in plants and microbes where they will interact with metal ions in
environment here ensuring the transport of metal ions to cytosol.
And by the metal-cochaperones these are transferred
to suitable receptor proteins. Plants respond high levels of heavy metals by
synthesizing (Abbaszadeh-Dahaji et
al., 2016; Ayangbenro
and Babalola, 2017). Heavy metals are being accumulated in soil through
sewage disposal and the industrial wastes. Among various traditional soil
remediation technologies phytoremediation use to
clean up the metal contaminated sites has increasing the attention as
co-friendly as well as inexpensive. Improvement of metal accretion plants
traits like genetic engineering necessary perspectives of certain many
biological processes implemented in it by roots from the soil sap and then
transferred to shoots. Metal contaminated soil by phytoremediation
can be done by different forms of genetically engineered rhizobacteria
(Canli et al.,
2001; Dodd et al., 2010; Joshi and Juwarkar, 2009). Phytoremediation is an
eco-friendly and the emerging technology that has gained the wide acceptance by,
any regulatory authorities. Its use is limited time taken to achieve the clean-up
goal (Cuypers et al.,
2011; Lahori et al., 2017; Li et al., 2015). Currently it is the area of the active research in
plant biology. Number of metal accumulated by plants has been identified as the
potential candidates to Phytoremediate the metal
polluted soil. Various types of strategies have been applied to generate the
plants that are help to grow in environmental conditions and to transfer the
number of metals (Dubey, 2010; Halder and Chakrabartty, 1993). The use of genetic engineering helps to modify the
plant for enhanced efficacy of phytoremediation
strategies. Plants are involved in this process and there are many chances of
food chain being distributed.
Conclusion
When evaluating
the rhizobacteria effects in phytoremediation
of toxic metals from contaminated soil, the process carried out both by
bacteria and plants while to protect plants from heavy metaltoxicity.
Certain bacteria use special developmental processes for phytoremediation
of heavy metals. Scientist are working to access the role of PGPR but still not
understanding the concept of phytoremediation. There
are of few questions which needs to be answered yet,
1.
There is need to
investigate the microbes induced changes in rhizosphere
of plant related to accumulation of metal, and also the contaminated soil.
2.
There is need to
quantify the effect of phytoremediation process on phytoavailability of heavy metals.
3.
There is need to
examine the accumulation and the distribution of toxic metals.
4.
The role played
by the bacteria from solution of soil in plant and the uptake of cadmium is
poorly understood yet.
5.
There is need to
understand the mobilization and the transfer of metals. Here we develop the
strategies and the optimization of phytoextraction
process. There is more need to understand the role of soil rhizobacteria
for phytoremediation.
Conflict of interest
The authors
declared absence of any conflict of interest.
References
De la Torre, F. R.,
Salibián, A., and Ferrari, L. (1999). Enzyme activities as biomarkers of
freshwater pollution: Responses of fish branchial (Na+ K)‐ATPase and liver transaminases. Environmental
Toxicology: An International Journal 14,
313-319.
Falkowski, P. G., and
Raven, J. A. (2013). "Aquatic photosynthesis," Princeton University
Press.
Felix, H. (1997). Field
trials for in situ decontamination of heavy metal polluted soils using crops of
metal‐accumulating plants. Zeitschrift
für Pflanzenernährung und Bodenkunde 160,
525-529.
Glick, B. R. (2012).
Plant growth-promoting bacteria: mechanisms and applications. Scientifica 2012.
Hughes, M. N., and
Poole, R. K. (1989). "Metals and Micro-organisms," Chapman and Hall.
Persello‐Cartieaux, F., Nussaume, L., and Robaglia, C. (2003). Tales from the
underground: molecular plant–rhizobacteria interactions. Plant, Cell & Environment 26,
189-199.
Pilon-Smits, E. (2005).
Phytoremediation. Annu. Rev. Plant Biol.
56, 15-39.
Requena, N., Jimenez,
I., Toro, M., and Barea, J. (1997). Interactions between plant‐growth‐promoting rhizobacteria (PGPR), arbuscular mycorrhizal fungi
and Rhizobium spp. in the rhizosphere of Anthyllis cytisoides, a model legume
for revegetation in mediterranean semi‐arid ecosystems. New Phytologist
136, 667-677.
Ross, S. M. (1994).
"Toxic metals in soil-plant systems," Wiley Chichester.
Tank, N., and Saraf, M.
(2009). Enhancement of plant growth and decontamination of nickel‐spiked soil using PGPR. Journal of
Basic Microbiology 49, 195-204.